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dc.contributor.authorAlves-Costa, Fernanda A.-
dc.contributor.authorDenovan-Wright, Eileen M.-
dc.contributor.authorThisse, Christine-
dc.contributor.authorThisse, Bernard-
dc.contributor.authorWright, Jonathan M.-
dc.identifier.citationFEBS Journal, v. 275, n. 13, p. 3325-3334, 2008.-
dc.description.abstractWe have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene contains four exons separated by three introns. The coding region of the gene and expressed sequence tags code for a polypeptide of 131 amino acids (14 kDa, pI 6.59). The putative zebrafish Fabp6 protein shared greatest sequence identity with human FABP6 (55.3%) compared to other orthologous mammalian FABPs and paralogous zebrafish Fabps. Phylogenetic analysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian FABP6s. The zebrafish fabp6 gene was assigned to linkage group (chromosome) 21 by radiation hybrid mapping. Conserved gene synteny was evident between the zebrafish fabp6 gene on chromosome 21 and the FABP6/Fabp6 genes on human chromosome 5, rat chromosome 10 and mouse chromosome 11. Zebrafish fabp6 transcripts were first detected in the distal region of the intestine of embryos at 72 h postfertilization. This spatial distribution remained constant to 7-day-old larvae, the last stage assayed during larval development. In adult zebrafish, fabp6 transcripts were detected by RT-PCR in RNA extracted from liver, heart, intestine, ovary and kidney (most likely adrenal tissue), but not in RNA from skin, brain, gill, eye or muscle. In situ hybridization of a fabp6 riboprobe to adult zebrafish sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intestine, and to a lesser extent in ovary and liver, a transcript distribution that is similar, but not identical, to that seen for the mammalian FABP6/Fabp6 gene. © 2008 The Authors.en
dc.subjectAdrenal gland-
dc.subjectConserved gene synteny-
dc.subjectIn situ hybridization-
dc.subjectLinkage group assignment-
dc.subjectfatty acid binding protein-
dc.subjectfatty acid binding protein 6-
dc.subjectamino acid sequence-
dc.subjectchromosome 11-
dc.subjectchromosome 21-
dc.subjectchromosome 5-
dc.subjectcontrolled study-
dc.subjectdevelopmental stage-
dc.subjectexpressed sequence tag-
dc.subjectgenetic linkage-
dc.subjectgenetic transcription-
dc.subjectin situ hybridization-
dc.subjectnucleotide sequence-
dc.subjectpriority journal-
dc.subjectradiation hybrid mapping-
dc.subjectreverse transcription polymerase chain reaction-
dc.subjecttissue distribution-
dc.subjectzebra fish-
dc.subjectAmino Acid Sequence-
dc.subjectBase Sequence-
dc.subjectDNA, Complementary-
dc.subjectFatty Acid-Binding Proteins-
dc.subjectGastrointestinal Hormones-
dc.subjectGene Expression Regulation, Developmental-
dc.subjectIn Situ Hybridization-
dc.subjectMolecular Sequence Data-
dc.subjectMultigene Family-
dc.subjectSequence Homology, Amino Acid-
dc.subjectTime Factors-
dc.subjectTissue Distribution-
dc.subjectTranscription, Genetic-
dc.subjectDanio rerio-
dc.titleSpatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio)en
dc.contributor.institutionDalhousie University-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversity of Virginia (UVA)-
dc.description.affiliationDepartment of Biology Dalhousie University, Halifax, NS B3H 4J1-
dc.description.affiliationDepartamento de Genética Instituto de Biociências UNESP - Universidade Estadual Paulista, CEP 18618, Botucatu, São Paulo-
dc.description.affiliationDepartment of Biology Dalhousie University, Halifax, NS-
dc.description.affiliationDepartment of Pharmacology, Halifax, NS-
dc.description.affiliationDepartment of Cell Biology University of Virginia Health Sciences Center, Charlottesville, VA-
dc.description.affiliationUnespDepartamento de Genética Instituto de Biociências UNESP - Universidade Estadual Paulista, CEP 18618, Botucatu, São Paulo-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofFEBS Journal-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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