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http://acervodigital.unesp.br/handle/11449/70657
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DC Field | Value | Language |
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dc.contributor.author | Conde, S. J. | - |
dc.contributor.author | Luvizotto, R. A M | - |
dc.contributor.author | Sibio, M. T. | - |
dc.contributor.author | Katayama, M. L H | - |
dc.contributor.author | Brentani, M. M. | - |
dc.contributor.author | Nogueira, Célia Regina | - |
dc.date.accessioned | 2014-05-27T11:23:42Z | - |
dc.date.accessioned | 2016-10-25T18:26:13Z | - |
dc.date.available | 2014-05-27T11:23:42Z | - |
dc.date.available | 2016-10-25T18:26:13Z | - |
dc.date.issued | 2008-12-01 | - |
dc.identifier | http://dx.doi.org/10.1007/BF03345650 | - |
dc.identifier.citation | Journal of Endocrinological Investigation, v. 31, n. 12, p. 1047-1051, 2008. | - |
dc.identifier.issn | 0391-4097 | - |
dc.identifier.uri | http://hdl.handle.net/11449/70657 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/70657 | - |
dc.description.abstract | Objectives: To examine the effects of triiodothyronine (T3), 17β-estradiol (E2), and tamoxifen (TAM) on transforming growth factor (TGF)-α gene expression in primary breast cancer cell cultures and interactions between the different treatments. Methods and results: Patients included in the study (no.=12) had been newly diagnosed with breast cancer. Fresh human breast carcinoma tissue was cut into 0.3-mm slices. These slices were placed in six 35-mm dishes on 2-ml organ culture medium. Dishes received the following treatments: dish 1: ethanol; dish 2: T3; dish 3: T3+TAM; dish 4: TAM; dish 5: E2; dish 6: E2+TAM. TGF-α mRNA content was normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA levels. All tissues included in this study were positive for estrogen receptor (ER) and thyroid hormone receptor expression. Treatment with T3 for 48 h significantly increased TGF-α mRNA levels compared to controls (15-fold), and concomitant treatment with TAM reduced expression to 3.4-fold compared to controls. When only TAM was added to the culture medium, TGF-α mRNA expression increased 5.3-fold, significantly higher than with all other treatment modalities. Conclusion: We demonstrate that TGF-α mRNA expression is more efficiently upregulated by T3 than E2. Concomitant treatment with TAM had a mitigating effect on the T3 effect, while E2 induced TGF-α upregulation. Our findings show some similarities between primary culture and breast cancer cell lines, but also some important differences: a) induction of TGF-α, a mitogenic protein, by TAM; b) a differential effect of TAM that may depend on relative expression of ER α and β; and c) supraphysiological doses of T3 may induce mitogenic signals in breast cancer tissue under conditions of low circulating E2. ©2008, Editrice Kurtis. | en |
dc.format.extent | 1047-1051 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | Breast cancer samples | - |
dc.subject | Estrogen | - |
dc.subject | Tamoxifen | - |
dc.subject | TGF-α | - |
dc.subject | Triiodothyronine | - |
dc.subject | cyclin D1 | - |
dc.subject | estradiol | - |
dc.subject | estrogen receptor alpha | - |
dc.subject | estrogen receptor beta | - |
dc.subject | glyceraldehyde 3 phosphate dehydrogenase | - |
dc.subject | liothyronine | - |
dc.subject | messenger RNA | - |
dc.subject | tamoxifen | - |
dc.subject | thyroid hormone receptor | - |
dc.subject | transforming growth factor alpha | - |
dc.subject | adult | - |
dc.subject | aged | - |
dc.subject | breast carcinoma | - |
dc.subject | cancer cell culture | - |
dc.subject | clinical article | - |
dc.subject | controlled study | - |
dc.subject | culture medium | - |
dc.subject | drug mechanism | - |
dc.subject | female | - |
dc.subject | gene expression regulation | - |
dc.subject | human | - |
dc.subject | human cell | - |
dc.subject | human tissue | - |
dc.subject | mitogenicity | - |
dc.subject | upregulation | - |
dc.subject | Adult | - |
dc.subject | Aged | - |
dc.subject | Aged, 80 and over | - |
dc.subject | Breast Neoplasms | - |
dc.subject | Carcinoma | - |
dc.subject | Cell Culture Techniques | - |
dc.subject | Down-Regulation | - |
dc.subject | Drug Interactions | - |
dc.subject | Estradiol | - |
dc.subject | Female | - |
dc.subject | Gene Expression Regulation, Neoplastic | - |
dc.subject | Humans | - |
dc.subject | Middle Aged | - |
dc.subject | Transforming Growth Factor alpha | - |
dc.subject | Tumor Cells, Cultured | - |
dc.title | Tamoxifen inhibits transforming growth factor-α gene expression in human breast carcinoma samples treated with triiodothyronine | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.description.affiliation | Division of Endocrinology and Metabolism Department of Medical Clinic UNESP, Rubião Junior, s/n, 18618-970 Botucatu, SP | - |
dc.description.affiliation | Division of Oncology Department of Radiology USP, São Paulo | - |
dc.description.affiliationUnesp | Division of Endocrinology and Metabolism Department of Medical Clinic UNESP, Rubião Junior, s/n, 18618-970 Botucatu, SP | - |
dc.identifier.doi | 10.1007/BF03345650 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of Endocrinological Investigation | - |
dc.identifier.scopus | 2-s2.0-63049106959 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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