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dc.contributor.authorAraújo, Danielle B.-
dc.contributor.authorLangoni, Hélio-
dc.contributor.authorAlmeida, Marilene F.-
dc.contributor.authorMegid, Jane-
dc.date.accessioned2014-05-27T11:23:43Z-
dc.date.accessioned2016-10-25T18:26:18Z-
dc.date.available2014-05-27T11:23:43Z-
dc.date.available2016-10-25T18:26:18Z-
dc.date.issued2008-12-01-
dc.identifierhttp://dx.doi.org/10.1186/1756-0500-1-17-
dc.identifier.citationBMC Research Notes, v. 1.-
dc.identifier.issn1756-0500-
dc.identifier.urihttp://hdl.handle.net/11449/70693-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/70693-
dc.description.abstractBackground. The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed materials. Additionally, molecular techniques have been used for epidemiological studies and to gain a better knowledge of viral epidemiology. Findings. The aim of this work was to evaluate the RT-PCR and hnRT-PCR for rabies virus detection in original tissues stored at -20°C for different periods considering their use for rabies virus detection in stored and decomposed samples. RT-PCR and hnRT-PCR were evaluated in 151 brain samples from different animal species, thawed and left at room temperature for 72 hours for decomposition. The RT-PCR and hnRT-PCR results were compared with previous results from Direct Fluorescent Antibody Test and Mouse Inoculation Test. From the 50 positive fresh samples, 26 (52%) were positive for RT-PCR and 45 (90%) for hnRT-PCR. From the 48 positive decomposed samples, 17 (34, 3%) were positive for RT-PCR and 36 (75%) for hnRT-PCR. No false-positives results were found in the negatives samples evaluated to the molecular techniques. Conclusion. These results show that the hnRT-PCR was more sensitive than RT-PCR, and both techniques presented lower sensibility in decomposed samples. The hnRT-PCR demonstrated efficacy in rabies virus detection in stored and decomposed materials suggesting it's application for rabies virus retrospective epidemiological studies. © 2008 Arajo et al; licensee BioMed Central Ltd.en
dc.language.isoeng-
dc.sourceScopus-
dc.subjectAnimalia-
dc.subjectRabies virus-
dc.titleHeminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samplesen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionCCZ-
dc.description.affiliationUNESP School of Veterinary and Animal Science Department of Veterinary Hygiene and Public Health, Botucatu, SP-
dc.description.affiliationCenter of Zoonosis Control CCZ, São Paulo, SP-
dc.description.affiliationUnespUNESP School of Veterinary and Animal Science Department of Veterinary Hygiene and Public Health, Botucatu, SP-
dc.identifier.doi10.1186/1756-0500-1-17-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-70449500458.pdf-
dc.relation.ispartofBMC Research Notes-
dc.identifier.scopus2-s2.0-70449500458-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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