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DC Field | Value | Language |
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dc.contributor.author | Araújo, Danielle B. | - |
dc.contributor.author | Langoni, Hélio | - |
dc.contributor.author | Almeida, Marilene F. | - |
dc.contributor.author | Megid, Jane | - |
dc.date.accessioned | 2014-05-27T11:23:43Z | - |
dc.date.accessioned | 2016-10-25T18:26:18Z | - |
dc.date.available | 2014-05-27T11:23:43Z | - |
dc.date.available | 2016-10-25T18:26:18Z | - |
dc.date.issued | 2008-12-01 | - |
dc.identifier | http://dx.doi.org/10.1186/1756-0500-1-17 | - |
dc.identifier.citation | BMC Research Notes, v. 1. | - |
dc.identifier.issn | 1756-0500 | - |
dc.identifier.uri | http://hdl.handle.net/11449/70693 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/70693 | - |
dc.description.abstract | Background. The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed materials. Additionally, molecular techniques have been used for epidemiological studies and to gain a better knowledge of viral epidemiology. Findings. The aim of this work was to evaluate the RT-PCR and hnRT-PCR for rabies virus detection in original tissues stored at -20°C for different periods considering their use for rabies virus detection in stored and decomposed samples. RT-PCR and hnRT-PCR were evaluated in 151 brain samples from different animal species, thawed and left at room temperature for 72 hours for decomposition. The RT-PCR and hnRT-PCR results were compared with previous results from Direct Fluorescent Antibody Test and Mouse Inoculation Test. From the 50 positive fresh samples, 26 (52%) were positive for RT-PCR and 45 (90%) for hnRT-PCR. From the 48 positive decomposed samples, 17 (34, 3%) were positive for RT-PCR and 36 (75%) for hnRT-PCR. No false-positives results were found in the negatives samples evaluated to the molecular techniques. Conclusion. These results show that the hnRT-PCR was more sensitive than RT-PCR, and both techniques presented lower sensibility in decomposed samples. The hnRT-PCR demonstrated efficacy in rabies virus detection in stored and decomposed materials suggesting it's application for rabies virus retrospective epidemiological studies. © 2008 Arajo et al; licensee BioMed Central Ltd. | en |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | Animalia | - |
dc.subject | Rabies virus | - |
dc.title | Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | CCZ | - |
dc.description.affiliation | UNESP School of Veterinary and Animal Science Department of Veterinary Hygiene and Public Health, Botucatu, SP | - |
dc.description.affiliation | Center of Zoonosis Control CCZ, São Paulo, SP | - |
dc.description.affiliationUnesp | UNESP School of Veterinary and Animal Science Department of Veterinary Hygiene and Public Health, Botucatu, SP | - |
dc.identifier.doi | 10.1186/1756-0500-1-17 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | 2-s2.0-70449500458.pdf | - |
dc.relation.ispartof | BMC Research Notes | - |
dc.identifier.scopus | 2-s2.0-70449500458 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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