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DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
Background: Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases. Methods: In this investigation was evaluated the effect of a previous vaccination with DNA-HSP65 on diabetes development induced by Streptozotocin (STZ). C57BL/6 mice received three vaccine doses or the corresponding empty vector and were then injected with multiple low doses of STZ. Results: DNA-HSP65 vaccination protected mice from STZ induced insulitis and this was associated with higher production of IL-10 in spleen and also in the islets. This protective effect was also concomitant with the appearance of a regulatory cell population in the spleen and a decreased infiltration of the islets by T CD8+ lymphocytes. The vector (DNAv) also determined immunomodulation but its protective effect against insulitis was very discrete. Conclusion: The data presented in this study encourages a further investigation in the regulatory potential of the DNA-HSP65 construct. Our findings have important implications for the development of new immune therapy strategies to combat autoimmune diseases. © 2009 Santos et al; licensee BioMed Central Ltd.
Issue Date: 
Journal of Immune Based Therapies and Vaccines, v. 7, p. 4-.
Time Duration: 
  • CD103 antigen
  • CD4 antigen
  • CD8 antigen
  • cytotoxic T lymphocyte antigen 4
  • DNA vaccine
  • heat shock protein 65
  • interleukin 10
  • interleukin 2 receptor alpha
  • monoclonal antibody
  • streptozocin
  • tumor necrosis factor alpha
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • autoimmunity
  • CD8+ T lymphocyte
  • controlled study
  • cytokine production
  • DNA vector
  • immunomodulation
  • immunotherapy
  • insulitis
  • lymphocyte subpopulation
  • lymphocytic infiltration
  • male
  • mouse
  • Mycobacterium
  • nonhuman
  • pancreas islet
  • regulatory T lymphocyte
  • spleen
  • streptozocin diabetes
  • treatment outcome
Access Rights: 
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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