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http://acervodigital.unesp.br/handle/11449/71151
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DC Field | Value | Language |
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dc.contributor.author | Santos Junior, Rubens R. | - |
dc.contributor.author | Sartori, Alexandrina | - |
dc.contributor.author | Lima, Deison S. | - |
dc.contributor.author | Souza, Patrícia R.M. | - |
dc.contributor.author | Coelho-Castelo, Arlete A.M. | - |
dc.contributor.author | Bonato, Vânia L.D. | - |
dc.contributor.author | Silva, Célio L. | - |
dc.date.accessioned | 2014-05-27T11:23:58Z | - |
dc.date.accessioned | 2016-10-25T18:27:24Z | - |
dc.date.available | 2014-05-27T11:23:58Z | - |
dc.date.available | 2016-10-25T18:27:24Z | - |
dc.date.issued | 2009-09-15 | - |
dc.identifier | http://dx.doi.org/10.1186/1476-8518-7-4 | - |
dc.identifier.citation | Journal of Immune Based Therapies and Vaccines, v. 7, p. 4-. | - |
dc.identifier.issn | 1476-8518 | - |
dc.identifier.uri | http://hdl.handle.net/11449/71151 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/71151 | - |
dc.description.abstract | Background: Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases. Methods: In this investigation was evaluated the effect of a previous vaccination with DNA-HSP65 on diabetes development induced by Streptozotocin (STZ). C57BL/6 mice received three vaccine doses or the corresponding empty vector and were then injected with multiple low doses of STZ. Results: DNA-HSP65 vaccination protected mice from STZ induced insulitis and this was associated with higher production of IL-10 in spleen and also in the islets. This protective effect was also concomitant with the appearance of a regulatory cell population in the spleen and a decreased infiltration of the islets by T CD8+ lymphocytes. The vector (DNAv) also determined immunomodulation but its protective effect against insulitis was very discrete. Conclusion: The data presented in this study encourages a further investigation in the regulatory potential of the DNA-HSP65 construct. Our findings have important implications for the development of new immune therapy strategies to combat autoimmune diseases. © 2009 Santos et al; licensee BioMed Central Ltd. | en |
dc.format.extent | 4 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | CD103 antigen | - |
dc.subject | CD4 antigen | - |
dc.subject | CD8 antigen | - |
dc.subject | cytotoxic T lymphocyte antigen 4 | - |
dc.subject | DNA vaccine | - |
dc.subject | heat shock protein 65 | - |
dc.subject | interleukin 10 | - |
dc.subject | interleukin 2 receptor alpha | - |
dc.subject | monoclonal antibody | - |
dc.subject | streptozocin | - |
dc.subject | tumor necrosis factor alpha | - |
dc.subject | animal cell | - |
dc.subject | animal experiment | - |
dc.subject | animal model | - |
dc.subject | animal tissue | - |
dc.subject | autoimmunity | - |
dc.subject | CD8+ T lymphocyte | - |
dc.subject | controlled study | - |
dc.subject | cytokine production | - |
dc.subject | DNA vector | - |
dc.subject | immunomodulation | - |
dc.subject | immunotherapy | - |
dc.subject | insulitis | - |
dc.subject | lymphocyte subpopulation | - |
dc.subject | lymphocytic infiltration | - |
dc.subject | male | - |
dc.subject | mouse | - |
dc.subject | Mycobacterium | - |
dc.subject | nonhuman | - |
dc.subject | pancreas islet | - |
dc.subject | regulatory T lymphocyte | - |
dc.subject | spleen | - |
dc.subject | streptozocin diabetes | - |
dc.subject | treatment outcome | - |
dc.title | DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes | en |
dc.type | outro | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | University of São Paulo Ribeirão Preto Medical School Department of Biochemistry and Immunology, Ribeirão Preto, São Paulo | - |
dc.description.affiliation | Department of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo | - |
dc.description.affiliation | Bioscience Institute São Paulo State University, Botucatu, São Paulo | - |
dc.description.affiliationUnesp | Department of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo | - |
dc.description.affiliationUnesp | Bioscience Institute São Paulo State University, Botucatu, São Paulo | - |
dc.identifier.doi | 10.1186/1476-8518-7-4 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | 2-s2.0-70449432883.pdf | - |
dc.relation.ispartof | Journal of Immune Based Therapies and Vaccines | - |
dc.identifier.scopus | 2-s2.0-70449432883 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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