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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/71151
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dc.contributor.authorSantos Junior, Rubens R.-
dc.contributor.authorSartori, Alexandrina-
dc.contributor.authorLima, Deison S.-
dc.contributor.authorSouza, Patrícia R.M.-
dc.contributor.authorCoelho-Castelo, Arlete A.M.-
dc.contributor.authorBonato, Vânia L.D.-
dc.contributor.authorSilva, Célio L.-
dc.date.accessioned2014-05-27T11:23:58Z-
dc.date.accessioned2016-10-25T18:27:24Z-
dc.date.available2014-05-27T11:23:58Z-
dc.date.available2016-10-25T18:27:24Z-
dc.date.issued2009-09-15-
dc.identifierhttp://dx.doi.org/10.1186/1476-8518-7-4-
dc.identifier.citationJournal of Immune Based Therapies and Vaccines, v. 7, p. 4-.-
dc.identifier.issn1476-8518-
dc.identifier.urihttp://hdl.handle.net/11449/71151-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/71151-
dc.description.abstractBackground: Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases. Methods: In this investigation was evaluated the effect of a previous vaccination with DNA-HSP65 on diabetes development induced by Streptozotocin (STZ). C57BL/6 mice received three vaccine doses or the corresponding empty vector and were then injected with multiple low doses of STZ. Results: DNA-HSP65 vaccination protected mice from STZ induced insulitis and this was associated with higher production of IL-10 in spleen and also in the islets. This protective effect was also concomitant with the appearance of a regulatory cell population in the spleen and a decreased infiltration of the islets by T CD8+ lymphocytes. The vector (DNAv) also determined immunomodulation but its protective effect against insulitis was very discrete. Conclusion: The data presented in this study encourages a further investigation in the regulatory potential of the DNA-HSP65 construct. Our findings have important implications for the development of new immune therapy strategies to combat autoimmune diseases. © 2009 Santos et al; licensee BioMed Central Ltd.en
dc.format.extent4-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCD103 antigen-
dc.subjectCD4 antigen-
dc.subjectCD8 antigen-
dc.subjectcytotoxic T lymphocyte antigen 4-
dc.subjectDNA vaccine-
dc.subjectheat shock protein 65-
dc.subjectinterleukin 10-
dc.subjectinterleukin 2 receptor alpha-
dc.subjectmonoclonal antibody-
dc.subjectstreptozocin-
dc.subjecttumor necrosis factor alpha-
dc.subjectanimal cell-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectanimal tissue-
dc.subjectautoimmunity-
dc.subjectCD8+ T lymphocyte-
dc.subjectcontrolled study-
dc.subjectcytokine production-
dc.subjectDNA vector-
dc.subjectimmunomodulation-
dc.subjectimmunotherapy-
dc.subjectinsulitis-
dc.subjectlymphocyte subpopulation-
dc.subjectlymphocytic infiltration-
dc.subjectmale-
dc.subjectmouse-
dc.subjectMycobacterium-
dc.subjectnonhuman-
dc.subjectpancreas islet-
dc.subjectregulatory T lymphocyte-
dc.subjectspleen-
dc.subjectstreptozocin diabetes-
dc.subjecttreatment outcome-
dc.titleDNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetesen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversity of São Paulo Ribeirão Preto Medical School Department of Biochemistry and Immunology, Ribeirão Preto, São Paulo-
dc.description.affiliationDepartment of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo-
dc.description.affiliationBioscience Institute São Paulo State University, Botucatu, São Paulo-
dc.description.affiliationUnespDepartment of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo-
dc.description.affiliationUnespBioscience Institute São Paulo State University, Botucatu, São Paulo-
dc.identifier.doi10.1186/1476-8518-7-4-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-70449432883.pdf-
dc.relation.ispartofJournal of Immune Based Therapies and Vaccines-
dc.identifier.scopus2-s2.0-70449432883-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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