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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/71585
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dc.contributor.authorLopes, Rodrigo Antonio-
dc.contributor.authorCardoso, Tereza Cristina-
dc.contributor.authorLuvizotto, Maria Cecília Rui-
dc.contributor.authorAndrade, Alexandre Lima de-
dc.date.accessioned2014-05-27T11:24:38Z-
dc.date.accessioned2016-10-25T18:28:23Z-
dc.date.available2014-05-27T11:24:38Z-
dc.date.available2016-10-25T18:28:23Z-
dc.date.issued2010-03-01-
dc.identifierhttp://dx.doi.org/10.1111/j.1463-5224.2009.00753.x-
dc.identifier.citationVeterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010.-
dc.identifier.issn1463-5216-
dc.identifier.issn1463-5224-
dc.identifier.urihttp://hdl.handle.net/11449/71585-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/71585-
dc.description.abstractPurpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists.en
dc.format.extent69-75-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectC-Myc-
dc.subjectDog-
dc.subjectELISA-
dc.subjectEyelid tumors-
dc.subjectP53-
dc.subjectPCR-
dc.subjectDNA-
dc.subjectMyc protein-
dc.subjectprotein p53-
dc.subjectRNA-
dc.subjectanimal-
dc.subjectanimal disease-
dc.subjectdog-
dc.subjectdog disease-
dc.subjecteyelid tumor-
dc.subjectgene expression regulation-
dc.subjectgenetics-
dc.subjectmetabolism-
dc.subjectphysiology-
dc.subjectAnimals-
dc.subjectDog Diseases-
dc.subjectDogs-
dc.subjectEyelid Neoplasms-
dc.subjectGene Expression Regulation, Neoplastic-
dc.subjectProto-Oncogene Proteins c-myc-
dc.subjectTumor Suppressor Protein p53-
dc.subjectCanis familiaris-
dc.titleOccurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumorsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationPostgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.description.affiliationDepartment of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.description.affiliationDepartment of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.description.affiliationUnespPostgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.description.affiliationUnespDepartment of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.description.affiliationUnespDepartment of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP-
dc.identifier.doi10.1111/j.1463-5224.2009.00753.x-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Ophthalmology-
dc.identifier.scopus2-s2.0-77951605799-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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