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DC Field | Value | Language |
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dc.contributor.author | Lopes, Rodrigo Antonio | - |
dc.contributor.author | Cardoso, Tereza Cristina | - |
dc.contributor.author | Luvizotto, Maria Cecília Rui | - |
dc.contributor.author | Andrade, Alexandre Lima de | - |
dc.date.accessioned | 2014-05-27T11:24:38Z | - |
dc.date.accessioned | 2016-10-25T18:28:23Z | - |
dc.date.available | 2014-05-27T11:24:38Z | - |
dc.date.available | 2016-10-25T18:28:23Z | - |
dc.date.issued | 2010-03-01 | - |
dc.identifier | http://dx.doi.org/10.1111/j.1463-5224.2009.00753.x | - |
dc.identifier.citation | Veterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010. | - |
dc.identifier.issn | 1463-5216 | - |
dc.identifier.issn | 1463-5224 | - |
dc.identifier.uri | http://hdl.handle.net/11449/71585 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/71585 | - |
dc.description.abstract | Purpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists. | en |
dc.format.extent | 69-75 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | C-Myc | - |
dc.subject | Dog | - |
dc.subject | ELISA | - |
dc.subject | Eyelid tumors | - |
dc.subject | P53 | - |
dc.subject | PCR | - |
dc.subject | DNA | - |
dc.subject | Myc protein | - |
dc.subject | protein p53 | - |
dc.subject | RNA | - |
dc.subject | animal | - |
dc.subject | animal disease | - |
dc.subject | dog | - |
dc.subject | dog disease | - |
dc.subject | eyelid tumor | - |
dc.subject | gene expression regulation | - |
dc.subject | genetics | - |
dc.subject | metabolism | - |
dc.subject | physiology | - |
dc.subject | Animals | - |
dc.subject | Dog Diseases | - |
dc.subject | Dogs | - |
dc.subject | Eyelid Neoplasms | - |
dc.subject | Gene Expression Regulation, Neoplastic | - |
dc.subject | Proto-Oncogene Proteins c-myc | - |
dc.subject | Tumor Suppressor Protein p53 | - |
dc.subject | Canis familiaris | - |
dc.title | Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Postgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.description.affiliation | Department of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.description.affiliation | Department of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.description.affiliationUnesp | Postgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.description.affiliationUnesp | Department of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.description.affiliationUnesp | Department of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP | - |
dc.identifier.doi | 10.1111/j.1463-5224.2009.00753.x | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Veterinary Ophthalmology | - |
dc.identifier.scopus | 2-s2.0-77951605799 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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