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dc.contributor.authorMendonca, Leonardo Meneghin-
dc.contributor.authordos Santos, Graciela Cristina-
dc.contributor.authordos Santos, Raquel Alves-
dc.contributor.authorTakahashi, Catarina Satie-
dc.contributor.authorPires Bianchi, Maria de Lourdes-
dc.contributor.authorGreggi Antunes, Lusania Maria-
dc.identifier.citationHuman & Experimental Toxicology. London: Sage Publications Ltd, v. 29, n. 8, p. 635-643, 2010.-
dc.description.abstractA very appropriate method for antigenotoxicity evaluation of antioxidants is the comet assay, since this analytical method detects initial DNA lesions that are still subject to repair; in other words, lesions that are very associated to damages resulting from the generation and subsequent action of reactive species. However, a solid evaluation should be developed in order to avoid inexact interpretations. In our study, besides the association of curcumin with cisplatin, curcumin and cisplatin agents were also tested separately. Classical genotoxic compounds, when tested by the comet assay, present an increase in the nucleoid tail; however, the cisplatin treatment has resulted in a decrease of DNA migration. This was an expected effect, as the cross-links between cisplatin and DNA decrease the DNA electrophoretic mobility. A similar effect was observed with the curcumin treatment, which decreased the nucleoid tail. Such effect was not expected and reinforced the necessity of including in the study, separate treatment groups with potentially antigenotoxic substances. The comet assay results have been analyzed using specific software for image analysis, as well as the classical visual analysis, and we have observed that the effect of decrease in DNA electrophoretic mobility was more easily observed when the data were analyzed by the software.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherSage Publications Ltd-
dc.sourceWeb of Science-
dc.subjectDNA damageen
dc.titleEvaluation of curcumin and cisplatin-induced DNA damage in PC12 cells by the alkaline comet assayen
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Alimentos & Nutr, São Paulo, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040903 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040903 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Alimentos & Nutr, São Paulo, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 08/53947-7-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofHuman & Experimental Toxicology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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