You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/72595
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGnanaprakasa, Tony J.-
dc.contributor.authorOyarzabal, Omar A.-
dc.contributor.authorOlsen, Eric V.-
dc.contributor.authorPedrosa, Valber A.-
dc.contributor.authorSimonian, Aleksandr L.-
dc.date.accessioned2014-05-27T11:25:57Z-
dc.date.accessioned2016-10-25T18:34:17Z-
dc.date.available2014-05-27T11:25:57Z-
dc.date.available2016-10-25T18:34:17Z-
dc.date.issued2011-08-10-
dc.identifierhttp://dx.doi.org/10.1016/j.snb.2011.04.037-
dc.identifier.citationSensors and Actuators, B: Chemical, v. 156, n. 1, p. 304-311, 2011.-
dc.identifier.issn0925-4005-
dc.identifier.urihttp://hdl.handle.net/11449/72595-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/72595-
dc.description.abstractDNA biosensors have gained increased attention over traditional diagnostic methods due to their fast and responsive operation and cost-effective design. The specificity of DNA biosensors relies on single-stranded oligonucleotide probes immobilized to a transduction platform. Here, we report the development of biosensors to detect the hippuricase gene (hipO) from Campylobacter jejuni using direct covalent coupling of thiol- and biotin-labeled single-stranded DNA (ssDNA) on both surface plasmon resonance (SPR) and diffraction optics technology (DOT, dotLab) transduction platforms. This is the first known report of the dotLab to detect targeted DNA. Application of 6-mercapto-1-hexanol as a spacer thiol for SPR gold surface created a self-assembled monolayer that removed unbound ssDNA and minimized non-specific detection. The detection limit of SPR sensors was shown to be 2.5 nM DNA while dotLab sensors demonstrated a slightly decreased detection limit of 5.0 nM (0.005 μM). It was possible to reuse the SPR sensor due to the negligible changes in sensor sensitivity (∼9.7 × 10 -7 ΔRU) and minimal damage to immobilized probes following use, whereas dotLab sensors could not be reused. Results indicated feasibility of optical biosensors for rapid and sensitive detection of the hipO gene of Campylobacter jejuni using specific ssDNA as a probe. © 2011 Elsevier B.V.en
dc.format.extent304-311-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCampylobacter jejuni-
dc.subjectDiffraction optics technology-
dc.subjectdotLab-
dc.subjecthipO gene-
dc.subjectLabel-free DNA detection-
dc.subjectSelf-assembled monolayers-
dc.subjectSPR-
dc.subjectSPREETA-
dc.subjectDiffraction optics-
dc.subjectLabel free-
dc.subjectBacteriophages-
dc.subjectBiosensors-
dc.subjectDiffraction-
dc.subjectElectron energy levels-
dc.subjectGenes-
dc.subjectOligonucleotides-
dc.subjectOrganic polymers-
dc.subjectProbes-
dc.subjectScaffolds-
dc.subjectSelf assembled monolayers-
dc.subjectSensors-
dc.subjectSurface plasmon resonance-
dc.subjectDNA-
dc.titleTethered DNA scaffolds on optical sensor platforms for detection of hipO gene from Campylobacter jejunien
dc.typeoutro-
dc.contributor.institutionAuburn University (AU)-
dc.contributor.institutionAlabama State University (ASU)-
dc.contributor.institutionKeesler Air Force Base-
dc.contributor.institutionUniversity of Arizona-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationMaterials Research and Education Center Samuel Ginn College of Engineering Auburn University, 275 Wilmore Laboratories, AL 36849-
dc.description.affiliationDepartment of Biological Sciences Alabama State University, Montgomery, AL 36104-
dc.description.affiliationClinical Research Laboratory 81st Medical Group, Keesler Air Force Base, Biloxi, MS 39534-
dc.description.affiliationDepartment of Materials Science and Engineering University of Arizona, Tucson, AZ-
dc.description.affiliationInstitute of Bioscience Department of Chemistry and Biochemistry UNESP, Botucatu, SP-
dc.description.affiliationUnespInstitute of Bioscience Department of Chemistry and Biochemistry UNESP, Botucatu, SP-
dc.identifier.doi10.1016/j.snb.2011.04.037-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofSensors and Actuators B: Chemical-
dc.identifier.scopus2-s2.0-79957849081-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.