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dc.contributor.authorSangalli-Leite, Fernanda-
dc.contributor.authorScorzoni, Liliana-
dc.contributor.authorCecilia Mesa-Arango, Ana-
dc.contributor.authorCasas, Celia-
dc.contributor.authorHerrero, Enrique-
dc.contributor.authorMendes-Giannini, Maria José Soares-
dc.contributor.authorLuis Rodriguez-Tudela, Juan-
dc.contributor.authorCuenca-Estrella, Manuel-
dc.contributor.authorZaragoza, Oscar-
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 5, p. 457-467, 2011.-
dc.description.abstractWe studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT assay and propidium iodide permeability). After 1 h of incubation, there were no viable colonies when the cells were exposed to AmB concentrations >= 1 mg/L. In the same conditions, the cells did not become permeable to propidium iodide, a phenomenon that was not observed until 3 h of incubation. When viability was measured in parallel using XTT assay, a result consistent with the CFUs was obtained, although we also observed a paradoxical effect in which at high AmB concentrations, a higher XTT reduction was measured than at intermediate AmB concentrations. This paradoxical effect was not observed after 3 h of incubation with AmB, and lack of XTT reduction was observed at AmB concentrations higher than 1 mg/L. When stained with dihydrofluorescein, AmB induced a strong intracellular oxidative burst. Consistent with oxidative damage, AmB induced protein carbonylation. Our results indicate that in C. neoformans, Amphotericin B causes intracellular damage mediated through the production of free radicals before damage on the cell membrane, measured by propidium iodide uptake. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.sponsorshipSpanish Ministry of Science and Innovation-
dc.description.sponsorshipCarolina Foundation-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectCryptococcus neoformansen
dc.subjectAmphotericin Ben
dc.subjectOxidative bursten
dc.subjectPropidium iodideen
dc.titleAmphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative bursten
dc.contributor.institutionInst Salud Carlos III-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniv Antioquia-
dc.contributor.institutionUniv Lleida-
dc.description.affiliationInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid 28220, Spain-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara SP, Lab Micol Clin, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUniv Antioquia, Grp Invest Dermatol, Medellin, Colombia-
dc.description.affiliationUniv Lleida, IRBLleida, Dept Basic Med Sci, Lleida 25008, Spain-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara SP, Lab Micol Clin, BR-14801902 Araraquara, SP, Brazil-
dc.description.sponsorshipIdSpanish MICINN: PCI2006-A7-0606-
dc.description.sponsorshipIdSpanish MICINN: SAF2008-03761-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofMicrobes and Infection-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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