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http://acervodigital.unesp.br/handle/11449/7327
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DC Field | Value | Language |
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dc.contributor.author | Bailao, Alexandre Melo | - |
dc.contributor.author | Nogueira, Sarah Veloso | - |
dc.contributor.author | Rondon Caixeta Bonfim, Sheyla Maria | - |
dc.contributor.author | de Castro, Kelly Pacheco | - |
dc.contributor.author | da Silva, Julhiany de Fatima | - |
dc.contributor.author | Mendes-Giannini, Maria José Soares | - |
dc.contributor.author | Pereira, Maristela | - |
dc.contributor.author | de Almeida Soares, Celia Maria | - |
dc.date.accessioned | 2014-05-20T13:23:57Z | - |
dc.date.accessioned | 2016-10-25T16:44:49Z | - |
dc.date.available | 2014-05-20T13:23:57Z | - |
dc.date.available | 2016-10-25T16:44:49Z | - |
dc.date.issued | 2012-04-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.resmic.2012.01.004 | - |
dc.identifier.citation | Research In Microbiology. Amsterdam: Elsevier B.V., v. 163, n. 3, p. 182-191, 2012. | - |
dc.identifier.issn | 0923-2508 | - |
dc.identifier.uri | http://hdl.handle.net/11449/7327 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/7327 | - |
dc.description.abstract | Paracoccidioidomycosis is caused by the dimorphic fungus Paracoccidioides brasiliensis. The extracellular matrix (ECM) plays an important role in regulation of cell adhesion, differentiation, migration and proliferation of cells. An in vitro binding assay of P. brasiliensis yeast cells adhering to type I collagen and fibronectin was performed in order to identify novel adhesins. Representational difference analysis (RDA) was employed to identify genes upregulated under adhesion-inducing conditions. Expressed sequence tags (ESTs) from cDNA libraries generated by the RDA technique were analyzed. Genes related to functional categories, such as metabolism, transcription, energy, protein synthesis and fate, cellular transport and biogenesis of cellular components were upregulated. Transcripts encoding the P. brasiliensis protein enolase (PbEno) and the high-affinity cooper transporter (PbCtr3) were identified and further characterized. The recombinant enolase (rPbEno) and a synthetic peptide designed for PbCtr3 were obtained and demonstrated to be able to bind ECM components. Immunofluorescence assays demonstrated that rPbEno specifically binds to the macrophage surface, reinforcing the role of this molecule in the P. brasiliensis interaction with host cells. In addition, upregulation of selected genes was demonstrated by qRT-PCR. In synthesis, the strategy can be useful in characterization of potential P. brasiliensis adhesins. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. | en |
dc.description.sponsorship | Financiadora de Estudos e Projetos (FINEP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 182-191 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Paracoccidioides brasiliensis | en |
dc.subject | Adhesin | en |
dc.subject | RDA | en |
dc.subject | Enolase | en |
dc.subject | Cooper transporter | en |
dc.title | Comparative transcriptome analysis of Paracoccidioides brasiliensis during in vitro adhesion to type I collagen and fibronectin: identification of potential adhesins | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Federal de Goiás (UFG) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Universidade Federal de Goiás (UFG), Inst Ciencias Biol, Lab Biol Mol, BR-74001970 Goiania, Go, Brazil | - |
dc.description.affiliation | UNESP, Fac Ciencias Farmaciut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, Fac Ciencias Farmaciut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil | - |
dc.description.sponsorshipId | FINEP: 0107055200 | - |
dc.description.sponsorshipId | FINEP: 0106121200 | - |
dc.description.sponsorshipId | CNPq: 471808/2006-7 | - |
dc.description.sponsorshipId | CNPq: 472947/2007-9 | - |
dc.identifier.doi | 10.1016/j.resmic.2012.01.004 | - |
dc.identifier.wos | WOS:000303230700004 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Research in Microbiology | - |
dc.identifier.orcid | 0000-0002-8059-0826 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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