You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/7327
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBailao, Alexandre Melo-
dc.contributor.authorNogueira, Sarah Veloso-
dc.contributor.authorRondon Caixeta Bonfim, Sheyla Maria-
dc.contributor.authorde Castro, Kelly Pacheco-
dc.contributor.authorda Silva, Julhiany de Fatima-
dc.contributor.authorMendes-Giannini, Maria José Soares-
dc.contributor.authorPereira, Maristela-
dc.contributor.authorde Almeida Soares, Celia Maria-
dc.date.accessioned2014-05-20T13:23:57Z-
dc.date.accessioned2016-10-25T16:44:49Z-
dc.date.available2014-05-20T13:23:57Z-
dc.date.available2016-10-25T16:44:49Z-
dc.date.issued2012-04-01-
dc.identifierhttp://dx.doi.org/10.1016/j.resmic.2012.01.004-
dc.identifier.citationResearch In Microbiology. Amsterdam: Elsevier B.V., v. 163, n. 3, p. 182-191, 2012.-
dc.identifier.issn0923-2508-
dc.identifier.urihttp://hdl.handle.net/11449/7327-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/7327-
dc.description.abstractParacoccidioidomycosis is caused by the dimorphic fungus Paracoccidioides brasiliensis. The extracellular matrix (ECM) plays an important role in regulation of cell adhesion, differentiation, migration and proliferation of cells. An in vitro binding assay of P. brasiliensis yeast cells adhering to type I collagen and fibronectin was performed in order to identify novel adhesins. Representational difference analysis (RDA) was employed to identify genes upregulated under adhesion-inducing conditions. Expressed sequence tags (ESTs) from cDNA libraries generated by the RDA technique were analyzed. Genes related to functional categories, such as metabolism, transcription, energy, protein synthesis and fate, cellular transport and biogenesis of cellular components were upregulated. Transcripts encoding the P. brasiliensis protein enolase (PbEno) and the high-affinity cooper transporter (PbCtr3) were identified and further characterized. The recombinant enolase (rPbEno) and a synthetic peptide designed for PbCtr3 were obtained and demonstrated to be able to bind ECM components. Immunofluorescence assays demonstrated that rPbEno specifically binds to the macrophage surface, reinforcing the role of this molecule in the P. brasiliensis interaction with host cells. In addition, upregulation of selected genes was demonstrated by qRT-PCR. In synthesis, the strategy can be useful in characterization of potential P. brasiliensis adhesins. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent182-191-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectParacoccidioides brasiliensisen
dc.subjectAdhesinen
dc.subjectRDAen
dc.subjectEnolaseen
dc.subjectCooper transporteren
dc.titleComparative transcriptome analysis of Paracoccidioides brasiliensis during in vitro adhesion to type I collagen and fibronectin: identification of potential adhesinsen
dc.typeoutro-
dc.contributor.institutionUniversidade Federal de Goiás (UFG)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversidade Federal de Goiás (UFG), Inst Ciencias Biol, Lab Biol Mol, BR-74001970 Goiania, Go, Brazil-
dc.description.affiliationUNESP, Fac Ciencias Farmaciut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUnespUNESP, Fac Ciencias Farmaciut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil-
dc.description.sponsorshipIdFINEP: 0107055200-
dc.description.sponsorshipIdFINEP: 0106121200-
dc.description.sponsorshipIdCNPq: 471808/2006-7-
dc.description.sponsorshipIdCNPq: 472947/2007-9-
dc.identifier.doi10.1016/j.resmic.2012.01.004-
dc.identifier.wosWOS:000303230700004-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofResearch in Microbiology-
dc.identifier.orcid0000-0002-8059-0826-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.