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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/73384
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dc.contributor.authorMimura, Kallyne K.-
dc.contributor.authorTedesco, Roberto C.-
dc.contributor.authorCalabrese, Katia S.-
dc.contributor.authorGil, Cristiane D.-
dc.contributor.authorOliani, Sonia M.-
dc.date.accessioned2014-05-27T11:26:51Z-
dc.date.accessioned2016-10-25T18:37:24Z-
dc.date.available2014-05-27T11:26:51Z-
dc.date.available2016-10-25T18:37:24Z-
dc.date.issued2012-06-15-
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382303/-
dc.identifier.citationMolecular Vision, v. 18, p. 1583-1593.-
dc.identifier.issn1090-0535-
dc.identifier.urihttp://hdl.handle.net/11449/73384-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/73384-
dc.description.abstractPurpose: The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. Methods: C57BL/6 female mice were infected using intravitreal injections of either 10 6 tachyzoites of Toxoplasma gondii (RH strain; T. gondii) or PBS only (control groups). After 24, 48, and 72 h, animals were sacrificed and their eyes were harvested for histopathological, immunohistochemical, and ultrastructural immunocytochemical analysis of ANXA1. Human retinal pigment epithelial (RPE) cells (ARPE-19) were infected in vitro with T. gondii and collected after 60, 120, 240 min, and 24 h. Results: Compared with non-infected eyes, an intense inflammatory response was observed in the anterior (24 h after infection) and posterior segments (72 h after infection) of the infected eye, characterized by neutrophil infiltration and by the presence of tachyzoites and their consequent destruction along with disorganization of normal retina architecture and RPE vacuolization. T. gondii infection was associated with a significant increase of ANXA1 expression in the neutrophils at 24, 48, and 72 h, and in the RPE at 48 and 72 h. In vitro studies confirmed an upregulation of ANXA1 levels in RPE cells, after 60 and 120 min of infection with T. gondii. Conclusions: The positive modulation of endogenous ANXA1 in the inflammatory and RPE cells during T. gondii infection suggests that this protein may serve as a therapeutic target in ocular toxoplasmosis. © 2012 Molecular Vision.en
dc.format.extent1583-1593-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectlipocortin 1-
dc.subjectanimal cell-
dc.subjectanimal experiment-
dc.subjectanimal model-
dc.subjectanimal tissue-
dc.subjectcell vacuole-
dc.subjectcontrolled study-
dc.subjectdisease association-
dc.subjecteye inflammation-
dc.subjectfemale-
dc.subjectin vitro study-
dc.subjectmouse-
dc.subjectneutrophil chemotaxis-
dc.subjectnonhuman-
dc.subjectpigment epithelium-
dc.subjectpriority journal-
dc.subjectprotein expression-
dc.subjectretina-
dc.subjecttachyzoite-
dc.subjectToxoplasma gondii-
dc.subjecttoxoplasmosis-
dc.subjectupregulation-
dc.subjectAnimals-
dc.subjectAnnexin A1-
dc.subjectAnterior Eye Segment-
dc.subjectEpithelial Cells-
dc.subjectFemale-
dc.subjectGene Expression Regulation-
dc.subjectHumans-
dc.subjectInterferon-gamma-
dc.subjectIntravitreal Injections-
dc.subjectMice-
dc.subjectMice, Inbred C57BL-
dc.subjectNeutrophil Infiltration-
dc.subjectPosterior Eye Segment-
dc.subjectRetinal Pigment Epithelium-
dc.subjectToxoplasma-
dc.subjectToxoplasmosis, Animal-
dc.subjectToxoplasmosis, Ocular-
dc.titleThe involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosisen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionInstituto Oswaldo Cruz/FIOCRUZ-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationFrom the Post-Graduation Structural and Functional Biology Federal University of São Paulo (UNIFESP), São Paulo, SP-
dc.description.affiliationDepartment of Morphology and Genetics Federal University of São Paulo (UNIFESP), São Paulo, SP-
dc.description.affiliationLaboratório de Imunomodulação e Protozoologia Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ-
dc.description.affiliationImmunomorphology Laboratory, Department of Biology Instituto de Biociências Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP), São José do Rio Preto, SP-
dc.description.affiliationUnespImmunomorphology Laboratory, Department of Biology Instituto de Biociências Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP), São José do Rio Preto, SP-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-84863323791.pdf-
dc.relation.ispartofMolecular Vision-
dc.identifier.scopus2-s2.0-84863323791-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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