You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDalmazzo, Leandro F. F.-
dc.contributor.authorSantana-Lemos, Barbara A.-
dc.contributor.authorJacomo, Rafael H.-
dc.contributor.authorGarcia, Aglair B.-
dc.contributor.authorRego, Eduardo M.-
dc.contributor.authorda Fonseca, Luiz M.-
dc.contributor.authorFalcao, Roberto P.-
dc.identifier.citationLeukemia Research. Oxford: Pergamon-Elsevier B.V. Ltd, v. 35, n. 5, p. 657-662, 2011.-
dc.description.abstractIndole-3-acetic acid (IAA), when oxidized by horseradish peroxidase (HRP), is transformed into cytotoxic molecules capable of inducing cell injury. The aim of this study was to test if, by targeting hematopoietic tumors with HRP-conjugated antibodies in association with IAA treatment, there is induction of apoptosis. We used two lineages of hematologic tumors: NB4, derived from acute promyelocytic leukemia (APL) and Granta-519 from mantle cell lymphoma (MCL). We also tested cells from 12 patients with acute myeloid leukemia (AML) and from 10 patients with chronic lymphocytic leukemia (CLL). HRP targeting was performed with anti-CD33 or anti-CD19 antibodies (depending on the origin of the cell), followed by incubation with goat anti-mouse antibody conjugated with HRP. Eight experimental groups were analyzed: control, HRP targeted, HRP targeted and incubated with 1, 5 and 10 mM IAA, and cells not HRP targeted but incubated with 1, 5 and 10 mM IAA. Apoptosis was analyzed by flow cytometry using annexin V-FITC and propidium iodide labeling. Results showed that apoptosis was dependent on the dose of IAA utilized, the duration of exposure to the prodrug and the origin of the neoplasia. Targeting HRP with antibodies was efficient in activating IAA and inducing apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherPergamon-Elsevier B.V. Ltd-
dc.sourceWeb of Science-
dc.subject3-Indole-acetic aciden
dc.subjectHorseradish peroxidaseen
dc.subjectAcute myeloid leukemiaen
dc.subjectChronic lymphocytic leukemiaen
dc.titleAntibody-targeted horseradish peroxidase associated with indole-3-acetic acid induces apoptosis in vitro in hematological malignanciesen
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv São Paulo, Med Sch Ribeirao Preto, Dept Internal Med, Div Hematol, BR-14048900 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, Med Sch Ribeirao Preto, Ctr Cell Based Therapy, BR-14048900 Ribeirao Preto, SP, Brazil-
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 05/58519-5-
dc.description.sponsorshipIdFAPESP: 05/04296-5-
dc.description.sponsorshipIdFAPESP: 98/14247-6-
dc.description.sponsorshipIdFAPESP: 57877-3/2008-
dc.description.sponsorshipIdCNPq: 573754/2009-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofLeukemia Research-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.