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dc.contributor.authorSchwartz-Filho, Humberto Osvaldo-
dc.contributor.authorBougas, Kostas-
dc.contributor.authorCoelho, Paulo G.-
dc.contributor.authorXue, Ying-
dc.contributor.authorHayashi, Mariko-
dc.contributor.authorFaeda, Rafael Silveira-
dc.contributor.authorMarcantonio, Rosemary Adriana Chierici-
dc.contributor.authorOno, Daisuke-
dc.contributor.authorKobayashi, Fumio-
dc.contributor.authorMustafa, Kamal-
dc.contributor.authorWennerberg, Ann-
dc.contributor.authorJimbo, Ryo-
dc.identifier.citationInternational Journal of Biomaterials.-
dc.description.abstractAim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 g/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface. © 2012 Humberto Osvaldo Schwartz-Filho et al.en
dc.titleThe effect of laminin-1-doped nanoroughened implant surfaces: Gene expression and morphological evaluationen
dc.contributor.institutionMalmö University-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionNew York University-
dc.contributor.institutionUniversity of Bergen-
dc.contributor.institutionGraduate School of Biomedical Sciences-
dc.description.affiliationDepartment of Prosthodontics Faculty of Odontology Malmö University, 205 06 Malmö-
dc.description.affiliationDepartment of Oral Diagnosis and Surgery School of Dentistry São Paulo State University, 01049-010 Araraquara, SP-
dc.description.affiliationDepartment of Biomaterials and Biomimetics New York University, New York, NY 10010-
dc.description.affiliationDepartment of Clinical Dentistry Center for Clinical Dental Research University of Bergen, 5020 Bergen-
dc.description.affiliationDivision of Applied Prosthodontics Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8102-
dc.description.affiliationUnespDepartment of Oral Diagnosis and Surgery School of Dentistry São Paulo State University, 01049-010 Araraquara, SP-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofInternational Journal of Biomaterials-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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