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http://acervodigital.unesp.br/handle/11449/74242
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DC Field | Value | Language |
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dc.contributor.author | Tezvergil-Mutluay, A. | - |
dc.contributor.author | Mutluay, M. | - |
dc.contributor.author | Seseogullari-Dirihan, R. | - |
dc.contributor.author | Agee, K. A. | - |
dc.contributor.author | Key, W. O. | - |
dc.contributor.author | Scheffel, D. L S | - |
dc.contributor.author | Breschi, L. | - |
dc.contributor.author | Mazzoni, A. | - |
dc.contributor.author | Tjäderhane, L. | - |
dc.contributor.author | Nishitani, Y. | - |
dc.contributor.author | Tay, F. R. | - |
dc.contributor.author | Pashley, D. H. | - |
dc.date.accessioned | 2014-05-27T11:27:30Z | - |
dc.date.accessioned | 2016-10-25T18:41:06Z | - |
dc.date.available | 2014-05-27T11:27:30Z | - |
dc.date.available | 2016-10-25T18:41:06Z | - |
dc.date.issued | 2013-01-01 | - |
dc.identifier | http://dx.doi.org/10.1177/0022034512466264 | - |
dc.identifier.citation | Journal of Dental Research, v. 92, n. 1, p. 87-91, 2013. | - |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.issn | 1544-0591 | - |
dc.identifier.uri | http://hdl.handle.net/11449/74242 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/74242 | - |
dc.description.abstract | This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We acid-etched experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 ± 0.8 ng ICTP and 0.6 ± 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices. © 2013 International & American Associations for Dental Research. | en |
dc.format.extent | 87-91 | - |
dc.language.iso | eng | - |
dc.source | Scopus | - |
dc.subject | bonding | - |
dc.subject | cathepsins | - |
dc.subject | collagen | - |
dc.subject | demineralized | - |
dc.subject | MMPs | - |
dc.subject | 4 aminophenylmercuriacetate | - |
dc.subject | 4-aminophenylmercuriacetate | - |
dc.subject | cathepsin | - |
dc.subject | collagen type 1 | - |
dc.subject | collagen type I trimeric cross linked peptide | - |
dc.subject | collagen type I trimeric cross-linked peptide | - |
dc.subject | collagenase | - |
dc.subject | cysteine proteinase inhibitor | - |
dc.subject | dipeptide | - |
dc.subject | drug derivative | - |
dc.subject | enzyme activator | - |
dc.subject | enzyme precursor | - |
dc.subject | ilomastat | - |
dc.subject | leucine | - |
dc.subject | matrix metalloproteinase | - |
dc.subject | matrix metalloproteinase inhibitor | - |
dc.subject | n [n (3 carboxyoxirane 2 carbonyl)leucyl]agmatine | - |
dc.subject | peptide | - |
dc.subject | peptide hydrolase | - |
dc.subject | phenylmercuric acetate | - |
dc.subject | phosphoric acid | - |
dc.subject | thiol reagent | - |
dc.subject | dentin | - |
dc.subject | drug antagonism | - |
dc.subject | drug effect | - |
dc.subject | enzymology | - |
dc.subject | human | - |
dc.subject | materials testing | - |
dc.subject | protein denaturation | - |
dc.subject | time | - |
dc.subject | Cathepsins | - |
dc.subject | Collagen Type I | - |
dc.subject | Collagenases | - |
dc.subject | Cysteine Proteinase Inhibitors | - |
dc.subject | Dentin | - |
dc.subject | Dipeptides | - |
dc.subject | Enzyme Activators | - |
dc.subject | Enzyme Precursors | - |
dc.subject | Humans | - |
dc.subject | Leucine | - |
dc.subject | Materials Testing | - |
dc.subject | Matrix Metalloproteinase Inhibitors | - |
dc.subject | Matrix Metalloproteinases | - |
dc.subject | Peptide Hydrolases | - |
dc.subject | Peptides | - |
dc.subject | Phenylmercuric Acetate | - |
dc.subject | Phosphoric Acids | - |
dc.subject | Protein Denaturation | - |
dc.subject | Sulfhydryl Reagents | - |
dc.subject | Time Factors | - |
dc.title | Effect of phosphoric acid on the degradation of human dentin matrix | en |
dc.type | outro | - |
dc.contributor.institution | University of Turku | - |
dc.contributor.institution | Georgia Health Sciences University | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | University of Trieste | - |
dc.contributor.institution | University of Bologna | - |
dc.contributor.institution | Oulu University Hospital | - |
dc.contributor.institution | Graduate School of Medicine, Dentistry and Pharmaceutical Sciences | - |
dc.contributor.institution | Unit of Bologna C/o IOR | - |
dc.description.affiliation | Adhesive Dentistry Research Group Institute of Dentistry University of Turku, Turku | - |
dc.description.affiliation | Finnish Doctoral Program in Oral Sciences (FINDOS) Institute of Dentistry University of Turku, 20520 Turku | - |
dc.description.affiliation | Department of Oral Biology College of Dental Medicine Georgia Health Sciences University, Augusta, GA | - |
dc.description.affiliation | Department of Orthodontics and Pediatric Dentistry Universidade Estadual Paulista-UNESP Araraquara Dental School, Araraquara, São Paulo | - |
dc.description.affiliation | Department of Biomedicine Unit of Dental Sciences and Biomaterials University of Trieste, Trieste | - |
dc.description.affiliation | Department of SAUandFAL University of Bologna, Bologna | - |
dc.description.affiliation | Institute of Dentistry University of Oulu Oulu University Hospital, Oulu | - |
dc.description.affiliation | Department of Operative Dentistry Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525 | - |
dc.description.affiliation | Department of Endodontics College of Dental Medicine Georgia Health Sciences University, Augusta, GA | - |
dc.description.affiliation | IGM-CNR Unit of Bologna C/o IOR, Bologna | - |
dc.description.affiliationUnesp | Department of Orthodontics and Pediatric Dentistry Universidade Estadual Paulista-UNESP Araraquara Dental School, Araraquara, São Paulo | - |
dc.identifier.doi | 10.1177/0022034512466264 | - |
dc.identifier.wos | WOS:000312209700015 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of Dental Research | - |
dc.identifier.scopus | 2-s2.0-84870925270 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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