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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/74483
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dc.contributor.authorBrito, Luciana G.-
dc.contributor.authorRocha, Rodrigo B.-
dc.contributor.authorBarbieri, Fábio da S.-
dc.contributor.authorRibeiro, Elisana S.-
dc.contributor.authorVendrami, Fabiano B.-
dc.contributor.authorSouza, Gislaine C.R.-
dc.contributor.authorGiglioti, Rodrigo-
dc.contributor.authorRegitano, Luciana C.A.-
dc.contributor.authorFalcoski, Thaís O.R.S.-
dc.contributor.authorTizioto, Polyana C.-
dc.contributor.authorOliveira, Márcia C.S.-
dc.date.accessioned2014-05-27T11:28:18Z-
dc.date.accessioned2016-10-25T18:43:18Z-
dc.date.available2014-05-27T11:28:18Z-
dc.date.available2016-10-25T18:43:18Z-
dc.date.issued2013-02-01-
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2012.08.001-
dc.identifier.citationTicks and Tick-borne Diseases, v. 4, n. 1-2, p. 78-82, 2013.-
dc.identifier.issn1877-959X-
dc.identifier.issn1877-9603-
dc.identifier.urihttp://hdl.handle.net/11449/74483-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/74483-
dc.description.abstractThe present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bovis in calves with ages from 4 to 12 months bred in 4 microregions each in the states of Rondônia and Acre. After the DNA was extracted from the samples, the infection in cattle was investigated by amplification of the rap1 gene from B. bovis. The DNA amplification results revealed a frequency of infection with B. bovis of 95.1% (272/286) in the samples from Rondônia and 96.1% (195/203) in those from Acre. The high frequency of B. bovis infection in the animals with ages from 4 to 12 months indicates a situation of enzootic stability in the regions studied. The infection rates are comparable to those detected by immunodiagnostic techniques in other endemic regions of Brazil. © 2012 Elsevier GmbH.en
dc.format.extent78-82-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectAmazonia-
dc.subjectBabesia bovis-
dc.subjectBrazil-
dc.subjectCattle-
dc.subjectEpidemiology-
dc.subjectRap1 protein-
dc.subjectblood clot-
dc.subjectblood sampling-
dc.subjectcalf (bovine)-
dc.subjectcattle-
dc.subjectcontrolled study-
dc.subjectDNA extraction-
dc.subjectDNA isolation-
dc.subjectgene-
dc.subjectgene amplification-
dc.subjectinfection rate-
dc.subjectnested polymerase chain reaction-
dc.subjectnonhuman-
dc.subjectnucleotide sequence-
dc.subjectpiroplasmosis-
dc.subjectpolymerase chain reaction-
dc.subjectpriority journal-
dc.subjectrap1 gene-
dc.subjectsensitivity and specificity-
dc.subjectserodiagnosis-
dc.subjectAnimals-
dc.subjectBabesiosis-
dc.subjectCattle Diseases-
dc.subjectDNA, Protozoan-
dc.subjectPolymerase Chain Reaction-
dc.subjectSensitivity and Specificity-
dc.subjectAnimalia-
dc.subjectBos-
dc.titleBabesia bovis infection in cattle in the southwestern Brazilian Amazonen
dc.typeoutro-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.contributor.institutionAgência de Defesa Sanitária Agrosilvopastoril do Estado de Rondônia-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)-
dc.description.affiliationEmbrapa Rondônia, Rodovia BR 364, km 5, 5, Caixa Postal 127, 76815-800, Porto Velho, RO-
dc.description.affiliationAgência de Defesa Sanitária Agrosilvopastoril do Estado de Rondônia, Porto Velho, RO-
dc.description.affiliationUniversidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP-
dc.description.affiliationEmbrapa Pecuária Sudeste, São Carlos, SP-
dc.description.affiliationUniversidade Estadual Júlio de Mesquita Filho, Araraquara, SP-
dc.description.affiliationUniversidade Federal de São Carlos, São Carlos, SP-
dc.description.affiliationUnespUniversidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP-
dc.description.affiliationUnespUniversidade Estadual Júlio de Mesquita Filho, Araraquara, SP-
dc.identifier.doi10.1016/j.ttbdis.2012.08.001-
dc.identifier.wosWOS:000316519000011-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofTicks and Tick-borne Diseases-
dc.identifier.scopus2-s2.0-84872612113-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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