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dc.contributor.authorKatiki, Luciana M.-
dc.contributor.authorFerreira, Jorge F.S.-
dc.contributor.authorGonzalez, Javier M.-
dc.contributor.authorZajac, Anne M.-
dc.contributor.authorLindsay, David S.-
dc.contributor.authorChagas, Ana Carolina S.-
dc.contributor.authorAmarante, Alessandro F.T.-
dc.identifier.citationVeterinary Parasitology, v. 192, n. 1-3, p. 218-227, 2013.-
dc.description.abstractAlthough tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0mg/mL to 25mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24h were determined and compared to the veterinary anthelmintic levamisole (8mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum, Rosa multiflora, and Quercus alba, while Rhus typhina had both HT and CT. The extracts with the lowest LC50 and LC90 concentrations, respectively, in the C. elegans assay were Q. alba (0.75 and 1.06mg/mL), R. typhina collected in 2007 (0.65 and 2.74mg/mL), A. rubrum (1.03 and 5.54mg/mL), and R. multiflora (2.14 and 8.70mg/mL). At the doses of 20 and 25mg/mL, HT-rich, or both CT- and HT-rich, extracts were significantly more lethal to adult C. elegans than extracts containing only CT. All extracts were high in antioxidant capacity, with ORAC values ranging from 1800μmoles to 4651μmoles of trolox equivalents/g, but ORAC did not correlate with anthelmintic activity. The total phenolics test had a positive and highly significant (r=0.826, p≤0.01) correlation with total hydrolyzable tannins. Plants used in this research are naturalized to the Appalachian edaphoclimatic conditions, but occur in temperate climate areas worldwide. They represent a rich, renewable, and unexplored source of tannins and antioxidants for grazing ruminants, whereas conventional CT-rich forages, such as L. cuneata, may be hard to establish and adapt to areas with temperate climate. Due to their high in vitro anthelmintic activity, antioxidant capacity, and their adaptability to non-arable lands, Q. alba, R. typhina, A. rubrum, and R. multiflora have a high potential to improve the health of grazing animals and must have their anthelmintic effects confirmed in vivo in both sheep and goats. © 2012.en
dc.subjectAnthelmintic plants-
dc.subjectAntioxidant capacity-
dc.subjectC. elegans-
dc.subjectCondensed tannins-
dc.subjectHydrolyzable tannins-
dc.subjectSmall ruminants-
dc.subjectTotal phenolics-
dc.subjectTree tannins-
dc.subjectAcer rubrum extract-
dc.subjectellagic acid-
dc.subjectgallic acid-
dc.subjectLespedeza cuneata extract-
dc.subjectphenol derivative-
dc.subjectplant extract-
dc.subjectQuercas alba extract-
dc.subjectRhus typhina extract-
dc.subjectRobinia pseudoacacia extract-
dc.subjectRosa multiflora extract-
dc.subjectSalix X sepulcralis extract-
dc.subjecttannin derivative-
dc.subjecttrolox C-
dc.subjectunclassified drug-
dc.subjectAcer rubrum-
dc.subjectanthelmintic activity-
dc.subjectantioxidant activity-
dc.subjectCaenorhabditis elegans-
dc.subjectconcentration (parameters)-
dc.subjectcontrolled study-
dc.subjecthigh performance liquid chromatography-
dc.subjectin vitro study-
dc.subjectLC 50-
dc.subjectLC 90-
dc.subjectlespedeza cuneata-
dc.subjectmedicinal plant-
dc.subjectplant leaf-
dc.subjectQuercas alba-
dc.subjectRhus typhina-
dc.subjectRobinia pseudoacacia-
dc.subjectRosa multiflora-
dc.subjectSalix X sepulcralis-
dc.subjectCapra hircus-
dc.subjectLespedeza cuneata-
dc.subjectOvis aries-
dc.subjectQuercus alba-
dc.subjectSalix x sepulcralis-
dc.titleAnthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacityen
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)-
dc.contributor.institutionAppalachian Farming Systems Research Center (USDA-ARS)-
dc.contributor.institutionVirginia Tech-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionNational Soil Erosion Research Lab-
dc.description.affiliationInstituto de Zootecnia (SAA-APTA), Rua Heitor Penteado 56, Nova Odessa, SP 13460-000-
dc.description.affiliationAppalachian Farming Systems Research Center (USDA-ARS), 1224 Airport Rd., Beaver, WV 25813-
dc.description.affiliationVirginia-Maryland Regional College of Veterinary Medicine Virginia Tech, Blacksburg, VA 24061-0442-
dc.description.affiliationEmbrapa Pecuária Sudeste, Rod. Washington Luiz km 234, São Carlos, SP-
dc.description.affiliationUNESP Universidade Estadual Paulista Departamento de Parasitologia, Botucatu, SP-
dc.description.affiliationUSDA ARS National Soil Erosion Research Lab, 275 S. Russell St., West Lafayette, IN 47907-
dc.description.affiliationUnespUNESP Universidade Estadual Paulista Departamento de Parasitologia, Botucatu, SP-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Parasitology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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