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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/74703
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dc.contributor.authorCândido-Bacani, Priscila de Matos-
dc.contributor.authorMori, Mateus Prates-
dc.contributor.authorCalvo, Tamara Regina-
dc.contributor.authorVilegas, Wagner-
dc.contributor.authorVaranda, Eliana Aparecida-
dc.contributor.authorDe Syllos Cólus, Ilce Mara-
dc.date.accessioned2014-05-27T11:28:35Z-
dc.date.accessioned2016-10-25T18:45:02Z-
dc.date.available2014-05-27T11:28:35Z-
dc.date.available2016-10-25T18:45:02Z-
dc.date.issued2013-03-01-
dc.identifierhttp://dx.doi.org/10.1080/15287394.2012.755941-
dc.identifier.citationJournal of Toxicology and Environmental Health - Part A: Current Issues, v. 76, n. 6, p. 354-362, 2013.-
dc.identifier.issn1528-7394-
dc.identifier.issn1087-2620-
dc.identifier.urihttp://hdl.handle.net/11449/74703-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/74703-
dc.description.abstractIsatin (1H-indole-2,3-dione) is a chemical found in various medicinal plant species and responsible for a broad spectrum of pharmacological and biological properties that may be beneficial to human health, as an anticonvulsant, antibacterial, antifungal, antiviral, and anticancer agent. The aim of the present study was to determine in vitro the cytotoxic, mutagenic, and apoptotic effects of isatin on CHO-K1 and HeLa cells using the MTT viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide), micronucleus (MN) test, apoptosis index, and nuclear division index (NDI). The 5 isatin concentrations evaluated in the mutagenicity and apoptosis tests were 0.5, 1, 5, 10, and 50 μM, selected through a preliminary MTT assay. Positive (doxorubicin, DXR) and negative (phosphate buffered saline, PBS) control groups were also included in the analysis. Isatin did not exert a mutagenic effect on CHO-K1 after 3 and 24 h of treatment or on HeLa cells after 24 h. However, 10 and 50 μM concentrations inhibited cell proliferation and promoted apoptosis in both CHO-K1 and HeLa cells. Data indicate that the cytotoxic, apoptotic, and antiproliferative effects of isatin were concentration independent and cell line independent. The authors thank Profa Dra Eiko Nakagawa Itano for the use the spectrophotometer and the Conselho Nacional para o Desenvolvimento Científico e Tecnológico for master's scholarships to P. M. Cândido-Bacani and grants to T. R. Calvo, W. Vilegas, E. A. Varanda and I. M. S. Cólus. The Conselho Nacional para o Desenvolvimento Científico e Tecnológico provided funding for this study. © 2013 Taylor & Francis Group, LLC.en
dc.format.extent354-362-
dc.language.isoeng-
dc.sourceScopus-
dc.subject3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide-
dc.subjectantineoplastic agent-
dc.subjectdoxorubicin-
dc.subjectisatin-
dc.subjectanimal cell-
dc.subjectantineoplastic activity-
dc.subjectapoptosis-
dc.subjectapoptosis index-
dc.subjectcells-
dc.subjectcontrolled study-
dc.subjectcytotoxicity-
dc.subjectHeLa cell-
dc.subjecthuman-
dc.subjecthuman cell-
dc.subjectin vitro study-
dc.subjectmicronucleus test-
dc.subjectmutagenicity-
dc.subjectnonhuman-
dc.subjectnuclear division index-
dc.subjectpriority journal-
dc.subjectAnimals-
dc.subjectApoptosis-
dc.subjectCell Division-
dc.subjectCell Nucleus-
dc.subjectCell Survival-
dc.subjectCHO Cells-
dc.subjectCricetinae-
dc.subjectCricetulus-
dc.subjectDNA, Neoplasm-
dc.subjectDose-Response Relationship, Drug-
dc.subjectFemale-
dc.subjectHeLa Cells-
dc.subjectHumans-
dc.subjectIsatin-
dc.subjectMicronuclei, Chromosome-Defective-
dc.subjectMicronucleus Tests-
dc.subjectMutagens-
dc.subjectPlant Extracts-
dc.subjectPlants, Medicinal-
dc.subjectTetrazolium Salts-
dc.subjectThiazoles-
dc.titleIn vitro assessment of the cytotoxic, apoptotic, and mutagenic potentials of Isatinen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of General Biology Biological Sciences Center Londrina State University, Londrina, PR-
dc.description.affiliationAraraquara Institute of Chemistry São Paulo State University, Araraquara, SP-
dc.description.affiliationDepartment of Biological Sciences Araraquara Faculty of Pharmaceutical Sciences São Paulo State University, Araraquara, SP-
dc.description.affiliationUnespAraraquara Institute of Chemistry São Paulo State University, Araraquara, SP-
dc.description.affiliationUnespDepartment of Biological Sciences Araraquara Faculty of Pharmaceutical Sciences São Paulo State University, Araraquara, SP-
dc.identifier.doi10.1080/15287394.2012.755941-
dc.identifier.wosWOS:000317245600002-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Toxicology and Environmental Health: Part A Current Issues-
dc.identifier.scopus2-s2.0-84876125299-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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