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dc.contributor.authorGregio, Ana P. B.-
dc.contributor.authorCano, Veridiana P. S.-
dc.contributor.authorAvaca, Juliana S.-
dc.contributor.authorValentini, Sandro Roberto-
dc.contributor.authorZanelli, Cleslei Fernando-
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 380, n. 4, p. 785-790, 2009.-
dc.description.abstractThe putative translation factor eIF5A is essential for cell viability and is highly conserved throughout evolution. Here, we describe genetic interactions between an eIF5A Mutant and a translation initiation mutant (eIF4E) or a translation elongation mutant (eEF2). Polysome profile analysis of single and double mutants revealed that mutation in eIF5A reduces polysome run-off, contrarily to translation initiation mutants. Moreover, the polysome profile of an eIF5A mutant alone is very similar to that of a translation elongation mutant. Furthermore, depletion of eIF5A causes a significant decrease in total protein synthesis and an increase of the average ribosome transit time. Finally, we, demonstrate that the formation of P bodies is inhibited in an eIF5A mutant, similarly to the effect of the translation elongation inhibitor cycloheximide. taken together, these results not only reinforce a role for eIF5A in translation but also strongly support a function for eIF5A in the elongation step of protein synthesis. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.publisherAcademic Press Inc. Elsevier B.V.-
dc.sourceWeb of Science-
dc.subjectTranslation elongationen
dc.subjectRibosome transit timeen
dc.titleeIF5A has a function in the elongation step of translation in yeasten
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationSão Paulo State Univ, Dept Biol Sci, Sch Pharmaceut Sci, UNESP,Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Dept Biol Sci, Sch Pharmaceut Sci, UNESP,Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiochemical and Biophysical Research Communications-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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