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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/74850
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dc.contributor.authorMoraes, Marli L.-
dc.contributor.authorLima, Lais R.-
dc.contributor.authorSilva, Robson R.-
dc.contributor.authorCavicchioli, Mauricio-
dc.contributor.authorRibeiro, Sidney J.L.-
dc.date.accessioned2014-05-27T11:28:40Z-
dc.date.accessioned2016-10-25T18:45:37Z-
dc.date.available2014-05-27T11:28:40Z-
dc.date.available2016-10-25T18:45:37Z-
dc.date.issued2013-03-19-
dc.identifierhttp://dx.doi.org/10.1021/la304404v-
dc.identifier.citationLangmuir, v. 29, n. 11, p. 3829-3834, 2013.-
dc.identifier.issn0743-7463-
dc.identifier.issn1520-5827-
dc.identifier.urihttp://hdl.handle.net/11449/74850-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/74850-
dc.description.abstractThe peptide NS5A-1 (PPLLESWKDPDYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide-silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix. © 2013 American Chemical Society.en
dc.format.extent3829-3834-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectAntigenic peptides-
dc.subjectHepatitis C virus-
dc.subjectImmobilization matrices-
dc.subjectLayer-by-layers-
dc.subjectNanostructured Films-
dc.subjectSecondary structures-
dc.subjectUV-vis absorptions-
dc.subjectVoltammetric sensor-
dc.subjectAntigens-
dc.subjectComposite films-
dc.subjectImmunosensors-
dc.subjectViruses-
dc.subjectPeptides-
dc.subjectantigen-
dc.subjectfibroin-
dc.subjectimmobilized protein-
dc.subjectnanomaterial-
dc.subjectNS 5 protein, hepatitis C virus-
dc.subjectNS-5 protein, hepatitis C virus-
dc.subjectpeptide fragment-
dc.subjectvirus protein-
dc.subjectamino acid sequence-
dc.subjectanimal-
dc.subjectchemistry-
dc.subjectgenetic procedures-
dc.subjectimmunology-
dc.subjectmethodology-
dc.subjectmolecular genetics-
dc.subjectAmino Acid Sequence-
dc.subjectAnimals-
dc.subjectBiosensing Techniques-
dc.subjectFibroins-
dc.subjectImmobilized Proteins-
dc.subjectMolecular Sequence Data-
dc.subjectNanostructures-
dc.subjectPeptide Fragments-
dc.subjectViral Nonstructural Proteins-
dc.titleImmunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured filmsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationInstitute of Chemistry São Paulo State University UNESP, CP355, Araraquara-SP, 14801-970-
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University UNESP, CP355, Araraquara-SP, 14801-970-
dc.identifier.doi10.1021/la304404v-
dc.identifier.wosWOS:000316168400037-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofLangmuir-
dc.identifier.scopus2-s2.0-84875304965-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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