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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/74860
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dc.contributor.authorHinfray, Nathalie-
dc.contributor.authorNóbrega, Rafael Henrique-
dc.contributor.authorCaulier, Morgane-
dc.contributor.authorBaudiffier, Damien-
dc.contributor.authorMaillot-Maréchal, Emmanuelle-
dc.contributor.authorChadili, Edith-
dc.contributor.authorPalluel, Olivier-
dc.contributor.authorPorcher, Jean-Marc-
dc.contributor.authorSchulz, Rüdiger-
dc.contributor.authorBrion, François-
dc.date.accessioned2014-05-27T11:28:42Z-
dc.date.accessioned2016-10-25T18:45:42Z-
dc.date.available2014-05-27T11:28:42Z-
dc.date.available2016-10-25T18:45:42Z-
dc.date.issued2013-03-21-
dc.identifierhttp://dx.doi.org/10.1530/JOE-12-0509-
dc.identifier.citationJournal of Endocrinology, v. 216, n. 3, p. 375-388, 2013.-
dc.identifier.issn0022-0795-
dc.identifier.issn1479-6805-
dc.identifier.urihttp://hdl.handle.net/11449/74860-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/74860-
dc.description.abstractOestrogens can affect expression of genes encoding steroidogenic enzymes in fish gonads. However, little information is available on their effects at the protein level. In this context, we first analysed the expression of key steroidogenic enzyme genes and proteins in zebrafish testis, paying attention also to other cell types than Leydig cells. Gene expression was analysed by quantitative PCR on fluorescence-activated cell-sorting fractions coupled or not to differential plating, while protein synthesis was studied by immunohistochemistry using specific antibodies against zebrafish Cyp17a1, Cyp19a1a and Cyp19a1b. Furthermore, we have evaluated the effect of oestrogen treatment (17β-oestradiol (E2), 10 nM) on the localization of these enzymes after 7 and 14 days of in vivo exposure in order to study how oestrogen-mediated modulation of their expression is linked to oestrogen effects on spermatogenesis. The major outcomes of this study are that Leydig cells express Cyp17a1 and Cyp19a1a, while testicular germ cells express Cyp17a1 and both, Cyp19a1a and Cyp19a1b. As regards Cyp17a1, both protein and mRNA seem to be quantitatively dominating in Leydig cells. Moreover, E2 exposure specifically affects only Leydig cell Cyp17a1 synthesis, preceding the disruption of spermatogenesis. The oestrogen-induced suppression of the androgen production capacity in Leydig cells is a major event in altering spermatogenesis, while germ cell steroidogenesis may have to be fuelled by precursors from Leydig cells. Further studies are needed to elucidate the functionality of steroidogenic enzymes in germ cells and their potential role in testicular physiology. © 2013 Society for Endocrinology.en
dc.format.extent375-388-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectEnzymes-
dc.subjectFacs-
dc.subjectImmunolocalization-
dc.subjectOestradiol-
dc.subjectSteroidogenic-
dc.subjectTestis-
dc.subjectZebrafish-
dc.subjectaromatase-
dc.subjectcyp17a1 protein-
dc.subjectcytochrome P450 17-
dc.subjectestradiol-
dc.subjectunclassified drug-
dc.subjectanimal cell-
dc.subjectanimal tissue-
dc.subjectcontrolled study-
dc.subjectdrug effect-
dc.subjectestrogen therapy-
dc.subjectgene expression-
dc.subjectgerm cell-
dc.subjectimmunohistochemistry-
dc.subjectLeydig cell-
dc.subjectnonhuman-
dc.subjectpolymerase chain reaction-
dc.subjectpriority journal-
dc.subjectprotein synthesis-
dc.subjectsynthesis-
dc.subjecttestis-
dc.subjectzebra fish-
dc.subjectAnimals-
dc.subjectAromatase-
dc.subjectEstradiol-
dc.subjectEstrogens-
dc.subjectGene Expression-
dc.subjectGerm Cells-
dc.subjectLeydig Cells-
dc.subjectMale-
dc.subjectSpermatogenesis-
dc.subjectSteroid 17-alpha-Hydroxylase-
dc.subjectZebrafish Proteins-
dc.titleCyp17a1 and cyp19a1 in the zebrafish testis are differentially affected by oestradiolen
dc.typeoutro-
dc.contributor.institutionPô le VIVA, Unité d'é cotoxicologie in vitro et in vivo-
dc.contributor.institutionUniversity of Utrecht-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationINERIS Direction des Risques Chroniques Pô le VIVA, Unité d'é cotoxicologie in vitro et in vivo, BP2, 60550 Verneuil-en-Halatte-
dc.description.affiliationReproductive Biology Group, Division of Developmental Biology Department of Biology, Science Faculty University of Utrecht, Padualaan 8, NL-3584 CH Utrecht-
dc.description.affiliationDepartment of Morphology Bioscience Institute São Paulo State University, RubiaoJr s/n, CEP 18618-000, Botucatu, São Paulo-
dc.description.affiliationUnespDepartment of Morphology Bioscience Institute São Paulo State University, RubiaoJr s/n, CEP 18618-000, Botucatu, São Paulo-
dc.identifier.doi10.1530/JOE-12-0509-
dc.identifier.wosWOS:000321246100012-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Endocrinology-
dc.identifier.scopus2-s2.0-84875081191-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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