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dc.contributor.authorZanetti Campanerut, Paula Aline-
dc.contributor.authorGhiraldi, Luciana Dias-
dc.contributor.authorEspinoza Spositto, Fernanda Luisa-
dc.contributor.authorSato, Daisy Nakamura-
dc.contributor.authorLeite, Clarice Queico Fujimura-
dc.contributor.authorHirata, Mario Hiroyuki-
dc.contributor.authorCrespo Hirata, Rosario Dominguez-
dc.contributor.authorCardoso, Rosilene Fressatti-
dc.identifier.citationJournal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 66, n. 5, p. 1044-1046, 2011.-
dc.description.abstractObjectives: The resazurin microtitre plate assay (REMA) was evaluated to determine the susceptibility of Mycobacterium tuberculosis to pyrazinamide, and was compared with the broth microdilution method (BMM), the absolute concentration method (ACM) and pyrazinamidase (PZase) determination.Methods: Thirty-four M. tuberculosis clinical isolates (26 susceptible and 8 resistant to pyrazinamide) and reference strains M. tuberculosis H37Rv ATCC 27294 and Mycobacterium bovis AN5 were tested.Results: REMA and BMM showed 100% specificity and sensitivity when compared with ACM; BMM, however, demanded more reading time. The PZase determination assay showed 87.50% and 100% sensitivity and specificity, respectively.Conclusions: All tested methods in this preliminary study showed excellent sensitivity and specificity for the determination of pyrazinamide susceptibility of M. tuberculosis, but REMA was faster, low-cost and easy to perform and interpret. Additional studies evaluating REMA for differentiating pyrazinamide-resistant and-susceptible M. tuberculosis should be conducted on an extended panel of clinical isolates.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherOxford University Press-
dc.sourceWeb of Science-
dc.subjectsusceptibility testingen
dc.titleRapid detection of resistance to pyrazinamide in Mycobacterium tuberculosis using the resazurin microtitre assayen
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)-
dc.contributor.institutionInstituto Adolfo Lutz (IAL)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationUniversidade Estadual de Maringá (UEM), Dept Clin Anal & Biomed, BR-87020900 Maringa, Parana, Brazil-
dc.description.affiliationUniversidade Estadual de Maringá (UEM), Postgrad Program Biosci Appl Pharm, BR-87020900 Maringa, Parana, Brazil-
dc.description.affiliationAdolfo Lutz Inst, BR-14085410 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Sch Pharmaceut Sci, BR-14801902 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo, Fac Pharmaceut Sci, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Sch Pharmaceut Sci, BR-14801902 São Paulo, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Antimicrobial Chemotherapy-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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