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dc.contributor.authorMachado, Daiane Cristina-
dc.contributor.authorMondini, Adriano-
dc.contributor.authorSantana, Vinicius dos Santos-
dc.contributor.authorKamiya Yonamine, Patricia Tiemi-
dc.contributor.authorChiaravalloti Neto, Francisco-
dc.contributor.authorde Andrade Zanotto, Paolo Marinho-
dc.contributor.authorNogueira, Mauricio Lacerda-
dc.identifier.citationIntervirology. Basel: Karger, v. 55, n. 6, p. 475-483, 2012.-
dc.description.abstractIntroduction: Culex flavivirus (CxFV) was first isolated in 2007 from Culex pipiens in Japan and then identified in several other countries. Characterization of the CxFV showed that all strains are related to the cell fusing agent virus. In this manuscript we report the first identification of CxFV in South America. Material and Methods: We have collected Culex sp. mosquitoes using BG-Sentinel traps and manual aspirators. They were pooled according to genus, species, sex and location. Viral RNA was extracted and multiplex nested PCR was performed to test the presence of Flavivirus. The positive samples were isolated in C6/36 cells and sequenced for phylogenetic analyses. Results: 265 female Culex mosquitoes pooled in 83 pools were tested with specific CxFV, Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV) primers. Our sequence data indicated maximum sequence similarity of 97% with CxFV. Discussion: In this study we report the circulation of CxFV in an urban setting where SLEV had previously caused an outbreak. In terms of public health, this is an important finding due to the assumption that the previous exposition of mosquitoes to CxFV might lessen the susceptibility of these mosquitoes to other flaviviruses. Copyright (C) 2012 S. Karger AG, Baselen
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipINCT-Dengue scholarship-
dc.sourceWeb of Science-
dc.subjectCulex flavivirusen
dc.subjectWest Nile virusen
dc.subjectPhylogenetic analysisen
dc.titleFirst Identification of Culex flavivirus (Flaviviridae) in Brazilen
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationFaculdade de Medicina de São José do Rio Preto (FAMERP), Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Araraquara, Brazil-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, Brazil-
dc.description.affiliationUniv São Paulo, São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Araraquara, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, Brazil-
dc.rights.accessRightsAcesso restrito-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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