You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorda Silva, Paulo-
dc.contributor.authorBarreto Santos, Adolfo Carlos-
dc.contributor.authorSato, Daisy Nakamura-
dc.contributor.authorSilva, Jaqueline Otero-
dc.contributor.authorCazentini Medeiros, Marta Ines-
dc.contributor.authorMachado Carneiro, Ana Maria-
dc.contributor.authorde Andrade Leite, Sergio Roberto-
dc.contributor.authorLeite, Clarice Queico Fujimura-
dc.identifier.citationBrazilian Journal of Infectious Diseases. Salvador: Contexto, v. 16, n. 5, p. 409-415, 2012.-
dc.description.abstractIntroduction: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics.Objective: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi.Methods: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification.Results: Among 78 Gram-positive and partially acid-fast bacilli isolated from the sputum of tuberculosis-suspected patients, 51 were phenotypically and genotypically characterized as R. equi based on literature data. Mycolic acid analysis showed that all suspected R. equi had compounds with a retention factor (R-f) between 0.4-0.5. Genotypic characterization indicated the presence of the choE gene 959 bp fragments in 51 isolates CAMP test positive. Twenty-two CAMP test negative isolates were negative for the choE gene. Five isolates presumptively identified as R. equi, CAMP test positive, were choE gene negative, and probably belonged to other bacterial species.Conclusions: The phenotypic and molecular techniques used constitute a good methodological tool to identify R. equi. (C) 2012 Elsevier Editora All rights reserved.en
dc.sourceWeb of Science-
dc.subjectPolymerase chain reactionen
dc.subjectRhodococcus equien
dc.subjectMycolic acidsen
dc.titlePhenotypic and genotypic characterization of Rhodococcus equi isolated from sputumen
dc.contributor.institutionInstituto Adolfo Lutz (IAL)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationAdolfo Lutz Inst, Nucleo Ciencias Biomed, Ctr Lab Reg IAL NCB CLR 6, BR-14085410 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho UNESP, Sch Pharmaceut Sci, Lab Micobacterias Prof Dr Hugo David, Araraquara, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho UNESP, Sch Pharmaceut Sci, Lab Micobacterias Prof Dr Hugo David, Araraquara, SP, Brazil-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofBrazilian Journal of Infectious Diseases-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.