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dc.contributor.authorFerreira, A. A. P.-
dc.contributor.authorColli, W.-
dc.contributor.authorAlves, M. J. M.-
dc.contributor.authorOliveira, D. R.-
dc.contributor.authorCosta, P. I.-
dc.contributor.authorGuell, A. G.-
dc.contributor.authorSanz, F.-
dc.contributor.authorBenedetti, Assis Vicente-
dc.contributor.authorYamanaka, Hideko-
dc.identifier.citationElectrochimica Acta. Oxford: Pergamon-Elsevier B.V., v. 51, n. 24, p. 5046-5052, 2006.-
dc.description.abstractThis present work reports on development of an amperometric immunosensor for the diagnosis of Chagas' disease using a specific glycoprotein of the trypomastigote surface, which belongs to the Tc85-11 protein family of Trypanosoma cruzi (T cruzi). An atomically flat gold surface on a silicon substrate and gold screen-printed electrodes were functionalized with cystatrine and later activated with glutaraldehyde (GA), which was used to form covalent bonds with the purified recombinant antigen (Tc85-11). The antigen reacts with the antibody from the serum, and the affinity reaction was monitored directly using atomic force microscopy or amperometry through a secondary antibody tagged to peroxidase (HRP). Surface imaging allowed to us to differentiate the modification steps and antigen-antibody interaction allowed to distinguish the affinity reactions. In the amperometric immunosensor, peroxidase catalyses the L-2 formation in the presence of hydrogen peroxide and potassium iodide, and the reduction current intensity was measured at a given potential with screen-printed electrodes. The immunosensor was applied to sera of chagasic patients and patients having different systemic diseases. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectChagas' diseasept
dc.subjectTc85-11 proteinpt
dc.subjectamperometric immunosensorpt
dc.titleInvestigation of the interaction between Tc85-11 protein and antibody anti-T cruzi by AFM and amperometric measurementsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniv Barcelona-
dc.description.affiliationUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil-
dc.description.affiliationUniv São Paulo, Inst Chem, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil-
dc.description.affiliationUniv Barcelona, CBEN, E-08028 Barcelona, Spain-
dc.description.affiliationUnespUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil-
dc.description.affiliationUnespUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofElectrochimica Acta-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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