You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/7556
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSkladal, P.-
dc.contributor.authorRiccardi, C. D.-
dc.contributor.authorYamanaka, Hideko-
dc.contributor.authorda Costa, P. I.-
dc.date.accessioned2014-05-20T13:24:24Z-
dc.date.accessioned2016-10-25T16:45:07Z-
dc.date.available2014-05-20T13:24:24Z-
dc.date.available2016-10-25T16:45:07Z-
dc.date.issued2004-05-01-
dc.identifierhttp://dx.doi.org/10.1016/j.jviromet.2004.01.005-
dc.identifier.citationJournal of Virological Methods. Amsterdam: Elsevier B.V., v. 117, n. 2, p. 145-151, 2004.-
dc.identifier.issn0166-0934-
dc.identifier.urihttp://hdl.handle.net/11449/7556-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/7556-
dc.description.abstractThe piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus (HCV) in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (four different sequences). Piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase-linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use. (C) 2004 Elsevier B.V. All rights reserved.en
dc.format.extent145-151-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectpiezoelectric biosensorpt
dc.subjecthepatitis C viruspt
dc.subjectRT-PCRpt
dc.titlePiezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virusen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionMasaryk Univ-
dc.description.affiliationUniv Estadual Paulista, Inst Chem, Dept Analyt Chem, BR-14801900 Araraquara, SP, Brazil-
dc.description.affiliationMasaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic-
dc.description.affiliationUniv Estadual Paulista, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Chem, Dept Analyt Chem, BR-14801900 Araraquara, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil-
dc.identifier.doi10.1016/j.jviromet.2004.01.005-
dc.identifier.wosWOS:000220770200006-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Virological Methods-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.