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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/75731
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dc.contributor.authorTata, Alessandra-
dc.contributor.authorSudano, Mateus J.-
dc.contributor.authorSantos, Vanessa G.-
dc.contributor.authorLandim-Alvarenga, Fernanda D.C.-
dc.contributor.authorFerreira, Christina R.-
dc.contributor.authorEberlin, Marcos N.-
dc.date.accessioned2014-05-27T11:29:48Z-
dc.date.accessioned2016-10-25T18:50:14Z-
dc.date.available2014-05-27T11:29:48Z-
dc.date.available2016-10-25T18:50:14Z-
dc.date.issued2013-07-01-
dc.identifierhttp://dx.doi.org/10.1002/jms.3231-
dc.identifier.citationJournal of Mass Spectrometry, v. 48, n. 7, p. 844-849, 2013.-
dc.identifier.issn1076-5174-
dc.identifier.issn1096-9888-
dc.identifier.urihttp://hdl.handle.net/11449/75731-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/75731-
dc.description.abstractIn pre-implantation embryos, lipids play key roles in determining viability, cryopreservation and implantation properties, but often their analysis is analytically challenging because of the few picograms of analytes present in each of them. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) allows obtaining individual phospholipid profiles of these microscopic organisms. This technique is sensitive enough to enable analysis of individual intact embryos and monitoring the changes in membrane lipid composition in the early stages of development serving as screening method for studies of biology and biotechnologies of reproduction. This article introduces an improved, more comprehensive MALDI-MS lipid fingerprinting approach that considerably increases the lipid information obtained from a single embryo. Using bovine embryos as a biological model, we have also tested optimal sample storage and handling conditions before the MALDI-MS analysis. Improved information at the molecular level is provided by the use of a binary matrix that enables phosphatidylcholines, sphingomyelins, phosphatidylserines, phosphatidylinositols and phosphoethanolamines to be detected via MALDI(±)-MS in both the positive and negative ion modes. An optimal MALDI-MS protocol for lipidomic monitoring of a single intact embryo is therefore reported with potential applications in human and animal reproduction, cell development and stem cell research. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.en
dc.format.extent844-849-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectbinary matrix-
dc.subjectembryo-
dc.subjectfingerprinting-
dc.subjectmatrix-assisted laser desorption-mass spectrometry (MALDI-MS)-
dc.subjectphospholipids-
dc.subjectBinary matrix-
dc.subjectMALDI-MS-
dc.subjectMatrix-assisted laser desorption/ionization mass spectrometry-
dc.subjectMembrane lipid composition-
dc.subjectMicroscopic organisms-
dc.subjectPre-implantation embryos-
dc.subjectOptimization-
dc.subjectPhospholipids-
dc.subjectStem cells-
dc.subjectMass spectrometry-
dc.subjectphosphatidylcholine-
dc.subjectphosphatidylinositol-
dc.subjectphosphatidylserine-
dc.subjectphosphoethanolamine-
dc.subjectsphingomyelin-
dc.subjectcell maturation-
dc.subjectfemale-
dc.subjectfertilization in vitro-
dc.subjectlipidomics-
dc.subjectmass spectrometry-
dc.subjectmatrix associated laser desorption ionization mass spectrometry-
dc.subjectnonhuman-
dc.subjectoocyte-
dc.subjectpriority journal-
dc.subjectreproduction-
dc.subjectstem cell-
dc.subjectstorage-
dc.titleOptimal single-embryo mass spectrometry fingerprintingen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationThoMSon Mass Spectrometry Laboratory University of Campinas, UNICAMP SP Institute of Chemistry, 13084-971, Campinas SP-
dc.description.affiliationSchool of Veterinary Medicine and Animal Science - FMVZ Department of Animal Reproduction and Veterinary Radiology São Paulo State University - UNESP, Botucatu, SP-
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal Science - FMVZ Department of Animal Reproduction and Veterinary Radiology São Paulo State University - UNESP, Botucatu, SP-
dc.identifier.doi10.1002/jms.3231-
dc.identifier.wosWOS:000321336000011-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Mass Spectrometry-
dc.identifier.scopus2-s2.0-84879961536-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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