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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/75747
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dc.contributor.authorGaldiano Jr., Renato Fernandes-
dc.contributor.authorde Macedo Lemos, Eliana Gertrudes-
dc.contributor.authorVendrame, Wagner Aparecido-
dc.date.accessioned2014-05-27T11:29:48Z-
dc.date.accessioned2016-10-25T18:50:16Z-
dc.date.available2014-05-27T11:29:48Z-
dc.date.available2016-10-25T18:50:16Z-
dc.date.issued2013-07-01-
dc.identifierhttp://dx.doi.org/10.1007/s11240-013-0304-4-
dc.identifier.citationPlant Cell, Tissue and Organ Culture, v. 114, n. 1, p. 139-148, 2013.-
dc.identifier.issn0167-6857-
dc.identifier.urihttp://hdl.handle.net/11449/75747-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/75747-
dc.description.abstractAn efficient cryopreservation protocol was developed for mature seeds of Oncidium flexuosum Sims. Seed morphology, protocorm formation, and early seedling development were also assessed. The effects of phloroglucinol and Supercool X-1000® as cryoprotectant additives in the vitrification solution were investigated. Dehydration using the plant vitrification solution 2 (PVS2) for 60 and 120 min prior to immersion in liquid nitrogen promoted the highest frequency of in vitro seed germination 6 weeks following culture on half-strength Murashige and Skoog (1/2 MS) medium. Mature seeds submitted to vitrification for 120 min in PVS2 and 1 % phloroglucinol at 0 °C enhanced germination by 68 %, whereas in PVS2 and 1 % Supercool X-1000® germination was just moderately enhanced (26 %). In vitro-germinating seedlings developed healthy shoots and roots without the use of plant growth regulators. After 6 months of growth, there were no differences between in vitro- and ex vitro-grown seedlings for various phenotypic characteristics, including shoot length, number of leaves, number and length of roots, and fresh and dry weight. Seedlings were transferred to greenhouse conditions and successfully acclimatized, further developing into normal plants with over 90 % survival. Comparative analysis of seedlings from control and vitrified seeds using flow cytometry indicated that no change in ploidy levels occurred as a result of cryopreservation, therefore maintaining seedlings genetic stability. In this study, vitrification with PVS2 for 120 min with the addition of 1 % phloroglucinol offers a simple, safe, and feasible protocol for cryopreservation of O. flexuosum mature seeds. © 2013 Springer Science+Business Media Dordrecht.en
dc.format.extent139-148-
dc.language.isoeng-
dc.sourceScopus-
dc.subjectFlow cytometry analysis-
dc.subjectIn vitro germination-
dc.subjectNative orchid-
dc.subjectPhenotypical characteristics-
dc.subjectVitrification-
dc.subjectComparative analysis-
dc.subjectGreenhouse conditions-
dc.subjectIn-vitro-
dc.subjectPlant growth regulators-
dc.subjectSeedling development-
dc.subjectBiological materials preservation-
dc.subjectCultivation-
dc.subjectFlow cytometry-
dc.subjectLiquid nitrogen-
dc.subjectPlants (botany)-
dc.subjectSupercooling-
dc.subjectSeed-
dc.subjectOncidium flexuosum-
dc.subjectOrchidaceae-
dc.titleCryopreservation, early seedling development, and genetic stability of Oncidium flexuosum Simsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversity of Florida-
dc.description.affiliationDepartment of Technology Univ Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane, s/n, Jaboticabal, 14884-900-
dc.description.affiliationTropical Research and Education Center University of Florida, 18905 SW 280th St, Homestead, FL, 33031-3314-
dc.description.affiliationDepartment of Technology Unesp, Univ Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane, s/n, Jaboticabal, 14884-900-
dc.description.affiliationUnespDepartment of Technology Univ Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane, s/n, Jaboticabal, 14884-900-
dc.description.affiliationUnespDepartment of Technology Unesp, Univ Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane, s/n, Jaboticabal, 14884-900-
dc.identifier.doi10.1007/s11240-013-0304-4-
dc.identifier.wosWOS:000320562300014-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofPlant Cell, Tissue and Organ Culture-
dc.identifier.scopus2-s2.0-84879230191-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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