Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/7587
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Silva, S. O. | - |
dc.contributor.author | Rodrigues, M. R. | - |
dc.contributor.author | Carvalho, SRQ | - |
dc.contributor.author | Catalani, L. H. | - |
dc.contributor.author | Campa, A. | - |
dc.contributor.author | Ximenes, Valdecir Farias | - |
dc.date.accessioned | 2014-05-20T13:24:27Z | - |
dc.date.accessioned | 2016-10-25T16:45:10Z | - |
dc.date.available | 2014-05-20T13:24:27Z | - |
dc.date.available | 2016-10-25T16:45:10Z | - |
dc.date.issued | 2004-10-01 | - |
dc.identifier | http://dx.doi.org/10.1111/j.1600-079X.2004.00149.x | - |
dc.identifier.citation | Journal of Pineal Research. Copenhagen: Blackwell Munksgaard, v. 37, n. 3, p. 171-175, 2004. | - |
dc.identifier.issn | 0742-3098 | - |
dc.identifier.uri | http://hdl.handle.net/11449/7587 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/7587 | - |
dc.description.abstract | N-1-acetyl-N-2-formyl-5-methoxykynuramine (AFMK) and N-1-acetyl-5-methoxykynuramine (AMK), two melatonin catabolites, have been described as potent antioxidants. We aimed to follow the kinetics of AFMK and AMK formation when melatonin is oxidized by phorbol myristate acetate (PMA) and lipopolysaccharide (LPS)-activated leukocytes. An HPLC-based method was used for AFMK and AMK determination in neutrophil and peripheral blood mononuclear cell cultures supernatants. Samples were separated isocratically on a C18 reverse-phase column using acetonitrile/H2O (25:75) as the mobile phase. AFMK was detected by fluorescence (excitation 340 nm and emission 460 nm) and AMK by UV-VIS absorbance (254 nm). Activation of neutrophils and mononuclear cells with PMA produces larger amounts of AFMK than activation with LPS, probably due to the lower levels of reactive oxygen species formation and myeloperoxidase (MPO) degranulation that occurs when cells are stimulated with LPS. The concentration of AMK found in the supernatant was about 5-10% (from 18-hr cultures) compared with AFMK. This result may reflect its reactivity. Indeed AMK, but not AFMK, is easily oxidized by activated neutrophils in a MPO and hydrogen peroxide-dependent reaction. In conclusion, we defined a simple procedure for the determination of AFMK and AMK in biological samples and demonstrated the capacity of leukocytes to oxidize melatonin and AMK. | en |
dc.format.extent | 171-175 | - |
dc.language.iso | eng | - |
dc.publisher | Blackwell Munksgaard | - |
dc.source | Web of Science | - |
dc.subject | melatonin | pt |
dc.subject | mononuclear cells | pt |
dc.subject | myeloperoxidase | pt |
dc.subject | N-1-acetyl-5-methoxykynuramine (AMK) | pt |
dc.subject | N-1-acetyl-N-2-formyl-5-methoxykynuramine (AFMK) neutrophils | pt |
dc.title | Oxidation of melatonin and its catabolites, N-1-acetyl-N (2)-formyl-5-methoxykynuramine and N-1-acetyl-5-methoxykynuramine, by activated leukocytes | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual de Londrina (UEL) | - |
dc.description.affiliation | UNESP, Fac Ciências Farmaceut Araraquara, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ São Paulo, Fac Ciências Farmaceut, Dept Anal Clin & Toxicol, São Paulo, Brazil | - |
dc.description.affiliation | Univ Estadual Londrina, Ctr Ciências Saude, Dept Patol Aplicada, Londrina, Brazil | - |
dc.description.affiliation | Univ São Paulo, Inst Quim, Dept Quim Fundamental, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP, Fac Ciências Farmaceut Araraquara, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil | - |
dc.identifier.doi | 10.1111/j.1600-079X.2004.00149.x | - |
dc.identifier.wos | WOS:000223709000005 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of Pineal Research | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.