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dc.contributor.authorMarsh, Sarah E.-
dc.contributor.authorPoulsen, Michael-
dc.contributor.authorGorosito, Norma B.-
dc.contributor.authorPinto-Tomás, Adrián-
dc.contributor.authorMasiulionis, Virginia E.-
dc.contributor.authorCurrie, Cameron R.-
dc.identifier.citationInternational Microbiology, v. 16, n. 1, p. 17-25, 2013.-
dc.description.abstractFungus-growing ants associate with multiple symbiotic microbes, including Actinobacteria for production of antibiotics. The best studied of these bacteria are within the genus Pseudonocardia, which in most fungus-growing ants are conspicuously visible on the external cuticle of workers. However, given that fungus-growing ants in the genus Atta do not carry visible Actinobacteria on their cuticle, it is unclear if this genus engages in the symbiosis with Pseudonocardia. Here we explore whether improving culturing techniques can allow for successful isolation of Pseudonocardia from Atta cephalotes leaf-cutting ants. We obtained Pseudonocardia from 9 of 11 isolation method/colony component combinations from all 5 colonies intensively sampled. The most efficient technique was bead-beating workers in phosphate buffer solution, then plating the suspension on carboxymethylcellulose medium. Placing these strains in a fungus-growing ant-associated Pseudonocardia phylogeny revealed that while some strains grouped with clades of Pseudonocardia associated with other genera of fungus-growing ants, a large portion of the isolates fell into two novel phylogenetic clades previously not identified from this ant-microbe symbiosis. Our findings suggest that Pseudonocardia may be associated with Atta fungus-growing ants, potentially internalized, and that localizing the symbiont and exploring its role is necessary to shed further light on the association.en
dc.subjectAtta leaf-cutter antsen
dc.titleAssociation between Pseudonocardia symbionts and Atta leaf-cutting ants suggested by improved isolation methodsen
dc.contributor.institutionUniv. of Wisconsin-Madison-
dc.contributor.institutionÉcole Normale Supérieure-
dc.contributor.institutionUniv. of Copenhagen-
dc.contributor.institutionNatl. Univ. of Quilmes-
dc.contributor.institutionUniv. of Buenos Aires-
dc.contributor.institutionUniv. of Costa Rica-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniv. of Wisconsin-
dc.description.affiliationDept. of Bacteriology Univ. of Wisconsin-Madison, Madison-
dc.description.affiliationInst. de Biologie École Normale Supérieure, Paris-
dc.description.affiliationSection for Ecol. and Evol. Dept. of Biology Univ. of Copenhagen, Copenhagen-
dc.description.affiliationCenter of Studies and Research Natl. Univ. of Quilmes, Bernal-
dc.description.affiliationDept. of Plant Production Univ. of Buenos Aires-
dc.description.affiliationDept. of Biochemistry School of Medicine Univ. of Costa Rica, San José-
dc.description.affiliationCenter of Research in Microscopic Structures Univ. of Costa Rica, San José-
dc.description.affiliationInst. of Biosciences UNESP Center of Studies of Social Insects, Sao Paulo, Rio Claro-
dc.description.affiliationUS Dept. of Energy Great Lakes Bioenergy Research Center Univ. of Wisconsin, Madison-
dc.description.affiliationUnespInst. of Biosciences UNESP Center of Studies of Social Insects, Sao Paulo, Rio Claro-
dc.description.sponsorshipIdNSF: DEB-747002-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofInternational Microbiology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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