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dc.contributor.authorAquino, I.-
dc.contributor.authorTsuboy, M. S F-
dc.contributor.authorMarcarini, J. C.-
dc.contributor.authorMantovani, M. S.-
dc.contributor.authorPerazzo, F. F.-
dc.contributor.authorMaistro, Edson Luis-
dc.identifier.citationGenetics and Molecular Research, v. 12, n. 3, p. 2517-2527, 2013.-
dc.description.abstractThe malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and genotoxicity, and expressions of the CASP3 and SOD1 genes in a cultured human hepatocellular liver carcinoma cell line (HepG2 cells) treated with artemisinin and artesunate. We tested concentrations of 2.5, 5, 7.5, 10, and 20 μg/mL of both substances with a resazurin cytotoxicity assay, and the concentrations used in the genotoxicity experiments (2.5, 5, and 10 μg/mL) and gene expression analysis (5 mg/mL) were determined. The results of the comet assay in cells treated with artemisinin and artesunate showed a significant dosedependent increase (P < 0.001) in the number of cells with DNA damage at all concentrations tested. However, the gene expression analysis revealed no significant change in expression of CASP3 or SOD1. Our data showed that although artemisinin and artesunate exhibited genotoxic effects in cultured HepG2 cells, they did not significantly alter expression of the CASP3 and SOD1 genes at the doses tested. ©FUNPEC-RP.en
dc.subjectCASP3 and SOD1 genes-
dc.subjectComet assay-
dc.subjectGene expression-
dc.subjectHepG2 cells-
dc.subjectcaspase 3-
dc.subjectcell DNA-
dc.subjectextracellular superoxide dismutase-
dc.subjectCasp3 gene-
dc.subjectcell count-
dc.subjectcell strain HepG2-
dc.subjectcomet assay-
dc.subjectconcentration response-
dc.subjectcontrolled study-
dc.subjectDNA damage-
dc.subjectdrug effect-
dc.subjectdrug screening-
dc.subjectgene expression-
dc.subjecthuman cell-
dc.subjectSOD1 gene-
dc.titleGenotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressionsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)-
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)-
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista, Botucatu, SP-
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista, Rio Claro, SP-
dc.description.affiliationLaboratório de Genética Toxicológica Universidade Estadual de Londrina, Londrina, PR-
dc.description.affiliationDepartamento de Ciências Exatas e da Terra Universidade Federal de São Paulo, Diadema, SP-
dc.description.affiliationDepartamento de Fonoaudiologia Universidade Estadual Paulista, Marília, SP-
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista, Botucatu, SP-
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista, Rio Claro, SP-
dc.description.affiliationUnespDepartamento de Fonoaudiologia Universidade Estadual Paulista, Marília, SP-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofGenetics and Molecular Research-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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