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dc.contributor.authorStafuzza, N. B.-
dc.contributor.authorGreco, A. J.-
dc.contributor.authorGrant, J. R.-
dc.contributor.authorAbbey, C. A.-
dc.contributor.authorGill, C. A.-
dc.contributor.authorRaudsepp, T.-
dc.contributor.authorSkow, L. C.-
dc.contributor.authorWomack, J. E.-
dc.contributor.authorRiggs, P. K.-
dc.contributor.authorAmaral, M. E J-
dc.identifier.citationAnimal Genetics, v. 44, n. 4, p. 369-376, 2013.-
dc.description.abstractThe major histocompatibility complex (MHC) in mammals codes for antigen-presenting proteins. For this reason, the MHC is of great importance for immune function and animal health. Previous studies revealed this gene-dense and polymorphic region in river buffalo to be on the short arm of chromosome 2, which is homologous to cattle chromosome 23. Using cattle-derived STS markers and a river buffalo radiation hybrid (RH) panel (BBURH5000), we generated a high-resolution RH map of the river buffalo MHC region. The buffalo MHC RH map (cR5000) was aligned with the cattle MHC RH map (cR 12000) to compare gene order. The buffalo MHC had similar organization to the cattle MHC, with class II genes distributed in two segments, class IIa and class IIb. Class IIa was closely associated with the class I and class III regions, and class IIb was a separate cluster. A total of 53 markers were distributed into two linkage groups based on a two-point LOD score threshold of ≥8. The first linkage group included 32 markers from class IIa, class I and class III. The second linkage group included 21 markers from class IIb. Bacterial artificial chromosome clones for seven loci were mapped by fluorescence in situ hybridization on metaphase chromosomes using single- and double-color hybridizations. The order of cytogenetically mapped markers in the region corroborated the physical order of markers obtained from the RH map and served as anchor points to align and orient the linkage groups. © 2012 The Authors, Animal Genetics © 2012 Stichting International Foundation for Animal Genetics.en
dc.subjectcomparative map-
dc.subjectfluorescence in situ hybridization-
dc.subjectmajor histocompatibility complex-
dc.subjectphysical map-
dc.subjectradiation hybrid map-
dc.subjectBacteria (microorganisms)-
dc.subjectBubalus bubalis-
dc.titleA high-resolution radiation hybrid map of the river buffalo major histocompatibility complex and comparison with BoLAen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionTexas AandM University-
dc.contributor.institutionUniversity of Alberta-
dc.description.affiliationDepartment of Biology UNESP - São Paulo State University IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, SP 15054-000-
dc.description.affiliationDepartment of Animal Science Texas AandM University, College Station TX 77843-
dc.description.affiliationDepartment of Agricultural, Food and Nutritional Science University of Alberta, Edmonton AB-
dc.description.affiliationDepartment of Veterinary Integrative Biosciences College of Veterinary Medicine Texas AandM University, College Station TX 77843-
dc.description.affiliationDepartment of Veterinary Pathobiology Texas AandM University, College Station TX 77843-
dc.description.affiliationUnespDepartment of Biology UNESP - São Paulo State University IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, SP 15054-000-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofAnimal Genetics-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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