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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/76274
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dc.contributor.authorde Oliveira, Jonatas Rafael-
dc.contributor.authorde Castro, Vinicius Carlos-
dc.contributor.authorVilela, Polyana das Graças Figueiredo-
dc.contributor.authorCamargo, Samira Esteves Afonso-
dc.contributor.authorCarvalho, Cláudio Antonio Talge-
dc.contributor.authorJorge, Antonio Olavo Cardoso-
dc.contributor.authorde Oliveira, Luciane Dias-
dc.date.accessioned2014-05-27T11:30:09Z-
dc.date.accessioned2016-10-25T18:52:37Z-
dc.date.available2014-05-27T11:30:09Z-
dc.date.available2016-10-25T18:52:37Z-
dc.date.issued2013-08-15-
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-13-208-
dc.identifier.citationBMC Complementary and Alternative Medicine, v. 13.-
dc.identifier.issn1472-6882-
dc.identifier.urihttp://hdl.handle.net/11449/76274-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/76274-
dc.description.abstractBackground: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1β in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL).Conclusions: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic. © 2013 de Oliveira et al.; licensee BioMed Central Ltd.en
dc.language.isoeng-
dc.sourceScopus-
dc.subjectCandida albicans-
dc.subjectCandida glabrata-
dc.subjectCandida tropicalis-
dc.subjectEquisetum arvense L-
dc.subjectGlycyrrhiza glabra L-
dc.subjectPunica granatum L-
dc.subjectStaphylococcus aureus-
dc.subjectStaphylococcus epidermidis-
dc.subjectStreptococcus mutans-
dc.subjectStryphnodendron barbatimam Mart-
dc.subjectBrazilian plant extract-
dc.subjectequisetum arvense extract-
dc.subjectGlycyrrhiza glabra extract-
dc.subjectinterleukin 1beta-
dc.subjectplant extract-
dc.subjectpomegranate extract-
dc.subjectStryphnodendron barbatimam extract-
dc.subjecttumor necrosis factor alpha-
dc.subjectunclassified drug-
dc.subjectanimal cell-
dc.subjectantimicrobial activity-
dc.subjectcell viability-
dc.subjectcolorimetry-
dc.subjectcontrolled study-
dc.subjectcytokine production-
dc.subjectcytotoxicity-
dc.subjectdentistry-
dc.subjectenzyme linked immunosorbent assay-
dc.subjectmacrophage-
dc.subjectmouse-
dc.subjectmouth flora-
dc.subjectnonhuman-
dc.subjectstimulation-
dc.subjectAnimals-
dc.subjectAnti-Bacterial Agents-
dc.subjectBacteria-
dc.subjectBrazil-
dc.subjectCell Line-
dc.subjectCell Survival-
dc.subjectMacrophages-
dc.subjectMice-
dc.subjectPlant Extracts-
dc.subjectPlants, Medicinal-
dc.subjectStomatognathic Diseases-
dc.subjectTumor Necrosis Factor-alpha-
dc.titleCytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistryen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology Institute of Science and Technology Univ Estadual Paulista/UNESP, Av. Engenheiro Francisco Jose Longo 777 - Jardim Sao Dimas, São José dos Campos, SP-
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis, Laboratory of Biochemistry and Pharmacology Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SP-
dc.description.affiliationDepartment of Restorative Dentistry Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SP-
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology Institute of Science and Technology Univ Estadual Paulista/UNESP, Av. Engenheiro Francisco Jose Longo 777 - Jardim Sao Dimas, São José dos Campos, SP-
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis, Laboratory of Biochemistry and Pharmacology Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SP-
dc.description.affiliationUnespDepartment of Restorative Dentistry Institute of Science and Technology Univ Estadual Paulista/UNESP, São José dos Campos, SP-
dc.identifier.doi10.1186/1472-6882-13-208-
dc.identifier.wosWOS:000323228600001-
dc.rights.accessRightsAcesso aberto-
dc.identifier.file2-s2.0-84882586629.pdf-
dc.relation.ispartofBMC Complementary and Alternative Medicine-
dc.identifier.scopus2-s2.0-84882586629-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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