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dc.contributor.authorEsteves, Jônatas C.-
dc.contributor.authorMarcantonio Júnior, Elcio-
dc.contributor.authorSouza Faloni, Ana P. de-
dc.contributor.authorRocha, Fernanda Regina G.-
dc.contributor.authorMarcantonio, Rosemary Adriana Chierici-
dc.contributor.authorWilk, Katarzyna-
dc.contributor.authorIntini, Giuseppe-
dc.identifier.citationJournal of Translational Medicine, v. 11, n. 1, 2013.-
dc.description.abstractBackground: Piezosurgery is an osteotomy system used in medical and dental surgery. Many studies have proven clinical advantages of piezosurgery in terms of quality of cut, maneuverability, ease of use, and safety. However, few investigations have tested its superiority over the traditional osteotomy systems in terms of dynamics of bone healing. Therefore, the aim of this study was to evaluate the dynamics of bone healing after osteotomies with piezosurgery and to compare them with those associated to traditional bone drilling.Methods: One hundred and ten rats were divided into two groups with 55 animals each. The animals were anesthetized and the tibiae were surgically exposed to create defects 2 mm in diameter by using piezosurgery (Piezo group) and conventional drilling (Drill group). Animals were sacrificed at 3, 7, 14, 30 and 60 days post-surgery. Bone samples were collected and processed for histological, histomorphometrical, immunohistochemical, and molecular analysis. The histological analysis was performed at all time points (n = 8) whereas the histomorphometrical analysis was performed at 7, 14, 30 and 60 days post-surgery (n = 8). The immunolabeling was performed to detect Vascular Endothelial Growth Factor (VEGF), Caspase-3 (CAS-3), Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), and Osteocalcin (OC) at 3, 7, and 14 days (n = 3). For the molecular analysis, animals were sacrificed at 3, 7 and 14 days, total RNA was collected, and quantification of the expression of 21 genes related to BMP signaling, Wnt signaling, inflammation, osteogenenic and apoptotic pathways was performed by qRT-PCR (n = 5).Results: Histologically and histomorphometrically, bone healing was similar in both groups with the exception of a slightly higher amount of newly formed bone observed at 30 days after piezosurgery (p < 0.05). Immunohistochemical and qRT-PCR analyses didn't detect significant differences in expression of all the proteins and most of the genes tested.Conclusions: Based on the results of our study we conclude that in a rat tibial bone defect model the bone healing dynamics after piezosurgery are comparable to those observed with conventional drilling. © 2013 Esteves et al.; licensee BioMed Central Ltd.en
dc.subjectBone drilling-
dc.subjectBone healing-
dc.subjectBone surgery-
dc.subjectOsteotomy system-
dc.titleDynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysisen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversity Center of Araraquara-UNIARA-
dc.contributor.institutionHarvard School of Dental Medicine - Harvard University-
dc.contributor.institutionHarvard Stem Cell Institute-
dc.description.affiliationDepartment of Diagnostic and Sugery Araraquara Dental School Univ Estadual Paulista - UNESP, Araraquara, São Paulo-
dc.description.affiliationDepartment of Diagnosis and Surgery - Periodontics Universidade Estadual Paulista (UNESP) Araraquara Dental School, Araraquara, SP-
dc.description.affiliationImplantology Post Graduation Course University Center of Araraquara-UNIARA, Araraquara, São Paulo-
dc.description.affiliationDepartment of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine - Harvard University, 188 Longwood Avenue, Boston, MA 02115 - REB 403-
dc.description.affiliationHarvard Stem Cell Institute, Cambridge, MA-
dc.description.affiliationUnespDepartment of Diagnostic and Sugery Araraquara Dental School Univ Estadual Paulista - UNESP, Araraquara, São Paulo-
dc.description.affiliationUnespDepartment of Diagnosis and Surgery - Periodontics Universidade Estadual Paulista (UNESP) Araraquara Dental School, Araraquara, SP-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofJournal of Translational Medicine-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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