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dc.contributor.authorHernandes, Rodrigo T.-
dc.contributor.authorDe la Cruz, Miguel A.-
dc.contributor.authorYamamoto, Denise-
dc.contributor.authorGirón, Jorge A.-
dc.contributor.authorGomes, Tânia A.T.-
dc.identifier.citationInfection and Immunity, v. 81, n. 10, p. 3793-3802, 2013.-
dc.description.abstractAtypical enteropathogenic Escherichia coli (aEPEC) strains are diarrheal pathogens that lack bundle-forming pilus production but possess the virulence-associated locus of enterocyte effacement. aEPEC strain 1551-2 produces localized adherence (LA) on HeLa cells; however, its isogenic intimin (eae) mutant produces a diffuse-adherence (DA) pattern. In this study, we aimed to identify the DA-associated adhesin of the 1551-2 eae mutant. Electron microscopy of 1551-2 identified rigid rod-like pili composed of an 18-kDa protein, which was identified as the major pilin subunit of type 1 pilus (T1P) by mass spectrometry analysis. Deletion of fimA in 1551-2 affected biofilm formation but had no effect on adherence properties. Analysis of secreted proteins in supernatants of this strain identified a 150-kDa protein corresponding to SslE, a type 2 secreted protein that was recently reported to be involved in biofilm formation of rabbit and human EPEC strains. However, neither adherence nor biofilm formation was affected in a 1551-2 sslE mutant. We then investigated the role of the EspA filament associated with the type 3 secretion system (T3SS) in DA by generating a double eae espA mutant. This strain was no longer adherent, strongly suggesting that the T3SS translocon is the DA adhesin. In agreement with these results, specific anti-EspA antibodies blocked adherence of the 1551-2 eae mutant. Our data support a role for intimin in LA, for the T3SS translocon in DA, and for T1P in biofilm formation, all of which may act in concert to facilitate host intestinal colonization by aEPEC strains. ©2013, American Society for Microbiology.en
dc.subjectbacterium adherence-
dc.subjectbacterium mutant-
dc.subjectbacterium pilus-
dc.subjectcontrolled study-
dc.subjectelectron microscopy-
dc.subjectenteropathogenic Escherichia coli-
dc.subjectgene deletion-
dc.subjectmass spectrometry-
dc.subjectpriority journal-
dc.subjectprotein analysis-
dc.subjectprotein secretion-
dc.subjecttype II secretion system-
dc.subjecttype III secretion system-
dc.titleDissection of the role of pili and type 2 and 3 secretion systems in adherence and biofilm formation of an atypical enteropathogenic escherichia coli strainen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)-
dc.contributor.institutionUniversity of Florida-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartamento de Microbiologia Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina, São Paulo-
dc.description.affiliationDepartment of Molecular Genetics and Microbiology Emerging Pathogens Institute University of Florida, Gainesville, FL-
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São Paulo-
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São Paulo-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofInfection and Immunity-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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