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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/7685
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dc.contributor.authorLeopoldino, A. M.-
dc.contributor.authorCanduri, F.-
dc.contributor.authorCabral, H.-
dc.contributor.authorJunqueira, M.-
dc.contributor.authorMarqui, A, B. T. de-
dc.contributor.authorApponi, L. H.-
dc.contributor.authorda Fonseca, I. O.-
dc.contributor.authorDomont, G. B.-
dc.contributor.authorSantos, D. S.-
dc.contributor.authorValentini, S.-
dc.contributor.authorBonilla-Rodriguez, G. O.-
dc.contributor.authorFossey, Marcelo Andrés-
dc.contributor.authorAzevedo, W. F. de-
dc.contributor.authorTajara, E. H.-
dc.date.accessioned2014-05-20T13:24:36Z-
dc.date.accessioned2016-10-25T16:45:19Z-
dc.date.available2014-05-20T13:24:36Z-
dc.date.available2016-10-25T16:45:19Z-
dc.date.issued2006-06-01-
dc.identifierhttp://dx.doi.org/10.1016/j.pep.2006.02.012-
dc.identifier.citationProtein Expression and Purification. San Diego: Academic Press Inc. Elsevier B.V., v. 47, n. 2, p. 614-620, 2006.-
dc.identifier.issn1046-5928-
dc.identifier.urihttp://hdl.handle.net/11449/7685-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/7685-
dc.description.abstractThe human cyclin-dependent kinase 9 (CDK9) protein was expressed in E coli BL21 using the pET23a vector at 30 degrees C. Several milligrams of protein were purified from soluble fraction using ionic exchange and ATP-affinity chromatography. The structural quality of recombinant CDK9 and the estimation of its secondary structure were obtained by circular dichroism. Structural models of CDK9 presented 26% of helices in agreement with the spectra by circular dichroism analysis. This is the first report on human CDK9 expression in Escherichia coli and structure analysis and provides the first step for the development of CDK9 inhibitors. (c) 2006 Elsevier B.V. All rights reserved.en
dc.format.extent614-620-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectCDK9pt
dc.subjectCâncerpt
dc.subjectAIDSpt
dc.subjectStructurept
dc.subjectdichroism analysispt
dc.subjectmolecular modelingpt
dc.subjectexpressionpt
dc.titleExpression, purification, and circular dichroism analysis of human CDK9en
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)-
dc.contributor.institutionPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)-
dc.description.affiliationFac Med Sao Jose do Rio Preto, Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUniv Fed Mato Grosso do Sul, Campo Grande, RS, Brazil-
dc.description.affiliationUniv Estadual Paulista, São Paulo, Brazil-
dc.description.affiliationUniv Fed Rio de Janeiro, Rede Proteom Rio, Rio de Janeiro, RJ, Brazil-
dc.description.affiliationUNESP, Fac Ciências Farmaceut, Araraquara, SP, Brazil-
dc.description.affiliationPUCRS, Ctr Pesquisas Biol Mol & Func, Porto Alegre, RS, Brazil-
dc.description.affiliationPUCRS, Fac Biociencias, Porto Alegre, RS, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, São Paulo, Brazil-
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut, Araraquara, SP, Brazil-
dc.identifier.doi10.1016/j.pep.2006.02.012-
dc.identifier.wosWOS:000238277000034-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofProtein Expression and Purification-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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