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DC Field | Value | Language |
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dc.contributor.author | Calgaro-Helena, A. F. | - |
dc.contributor.author | Devienne, K. F. | - |
dc.contributor.author | Rodrigues, T. | - |
dc.contributor.author | Dorta, D. J. | - |
dc.contributor.author | Raddi, MSG | - |
dc.contributor.author | Vilegas, Wagner | - |
dc.contributor.author | Uyemura, S. A. | - |
dc.contributor.author | Santos, A. C. | - |
dc.contributor.author | Curti, C. | - |
dc.date.accessioned | 2014-05-20T13:24:41Z | - |
dc.date.available | 2014-05-20T13:24:41Z | - |
dc.date.issued | 2006-06-10 | - |
dc.identifier | http://dx.doi.org/10.1016/j.cbi.2006.04.006 | - |
dc.identifier.citation | Chemico-biological Interactions. Clare: Elsevier B.V., v. 161, n. 2, p. 155-164, 2006. | - |
dc.identifier.issn | 0009-2797 | - |
dc.identifier.uri | http://hdl.handle.net/11449/7738 | - |
dc.description.abstract | Isolated mitochondria may undergo uncoupling, and in presence of Ca2+ at different conditions, a mitochondrial permeability transition (MPT) linked to protein,thiol oxidation, and demonstrated by CsA-sensitive mitochondrial swelling; these processes may cause cell death either by necrosis or by apoptosis. Isocoumarins isolated from the Brazilian plant Paepalanthus bromelioides (Eriocaulaceae) paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naptho(2,3c)pyran-1-one), 8,8'-paepalantine dimer, and vioxanthin were assayed at 1-50 mu M on isolated rat liver mitochondria, for respiration, MPT, protein thiol oxidation, and interaction with the mitochondrial membrane using 1,6-diphenyl-1,3,5-hexatriene (DPH). The isocoumarins did not significantly affect state 3 respiration of succinate-energized mitochondria; they did however, stimulate 4 respiration, indicating mitochondrial uncoupling. Induction of MPT and protein thiol oxidation were assessed in succinate-energized mitochondria exposed to 10 mu M Ca2+; inhibition of these processes was assessed in non-energized organelles in the presence of 300 mu M t-butyl hydroperoxide plus 500 mu M Ca2+. Only paepalantine was an effective MPT/protein thiol oxidation inducer, also releasing cytochrome c from mitochondria; the protein thiol oxidation, unlike mitochondrial swelling, was neither inhibited by CsA nor dependent on the presence of Ca2+. Vioxanthin was an effective inhibitor of MPT/protein thiol oxidation. All isocoumarins inserted deeply into the mitochondrial membrane, but only paepalantine dimer and vioxantin decreased the membrane's fluidity. A direct reaction with mitochondrial membrane protein thiols, involving an oxidation of these groups, is proposed to account for MPT induction by paepalantine, while a restriction of oxidation of these same thiol groups imposed by the decrease of membrane fluidity, is proposed to account for MPT inhibition by vioxanthin. (c) 2006 Published by Elsevier B.V.. | en |
dc.format.extent | 155-164 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | isocoumarins | pt |
dc.subject | paepalantine | pt |
dc.subject | liver mitochondria | pt |
dc.subject | uncoupling | pt |
dc.subject | mitochondrial permeability transition | pt |
dc.subject | permeability transition pore | pt |
dc.subject | protein thiol oxidation | pt |
dc.subject | mitochondrial membrane fluidity | pt |
dc.title | Effects of isocoumarins isolated from Paepalanthus bromelioides on mitochondria: Uncoupling, and induction/inhibition of mitochondrial permeability transition | en |
dc.type | outro | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ São Paulo, Fac Ciências Farmaceut, Dept Fis & Quim, BR-14040903 Ribeirao Preto, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, Inst Quim, Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, Fac Ciências Farmaceut, Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ São Paulo, Fac Ciências Farmaceut, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Quim, Araraquara, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Fac Ciências Farmaceut, Araraquara, SP, Brazil | - |
dc.identifier.doi | 10.1016/j.cbi.2006.04.006 | - |
dc.identifier.wos | WOS:000238606700007 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Chemico-biological Interactions | - |
dc.identifier.orcid | 0000-0003-3032-2556 | pt |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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