You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/7738
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCalgaro-Helena, A. F.-
dc.contributor.authorDevienne, K. F.-
dc.contributor.authorRodrigues, T.-
dc.contributor.authorDorta, D. J.-
dc.contributor.authorRaddi, MSG-
dc.contributor.authorVilegas, Wagner-
dc.contributor.authorUyemura, S. A.-
dc.contributor.authorSantos, A. C.-
dc.contributor.authorCurti, C.-
dc.date.accessioned2014-05-20T13:24:41Z-
dc.date.available2014-05-20T13:24:41Z-
dc.date.issued2006-06-10-
dc.identifierhttp://dx.doi.org/10.1016/j.cbi.2006.04.006-
dc.identifier.citationChemico-biological Interactions. Clare: Elsevier B.V., v. 161, n. 2, p. 155-164, 2006.-
dc.identifier.issn0009-2797-
dc.identifier.urihttp://hdl.handle.net/11449/7738-
dc.description.abstractIsolated mitochondria may undergo uncoupling, and in presence of Ca2+ at different conditions, a mitochondrial permeability transition (MPT) linked to protein,thiol oxidation, and demonstrated by CsA-sensitive mitochondrial swelling; these processes may cause cell death either by necrosis or by apoptosis. Isocoumarins isolated from the Brazilian plant Paepalanthus bromelioides (Eriocaulaceae) paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naptho(2,3c)pyran-1-one), 8,8'-paepalantine dimer, and vioxanthin were assayed at 1-50 mu M on isolated rat liver mitochondria, for respiration, MPT, protein thiol oxidation, and interaction with the mitochondrial membrane using 1,6-diphenyl-1,3,5-hexatriene (DPH). The isocoumarins did not significantly affect state 3 respiration of succinate-energized mitochondria; they did however, stimulate 4 respiration, indicating mitochondrial uncoupling. Induction of MPT and protein thiol oxidation were assessed in succinate-energized mitochondria exposed to 10 mu M Ca2+; inhibition of these processes was assessed in non-energized organelles in the presence of 300 mu M t-butyl hydroperoxide plus 500 mu M Ca2+. Only paepalantine was an effective MPT/protein thiol oxidation inducer, also releasing cytochrome c from mitochondria; the protein thiol oxidation, unlike mitochondrial swelling, was neither inhibited by CsA nor dependent on the presence of Ca2+. Vioxanthin was an effective inhibitor of MPT/protein thiol oxidation. All isocoumarins inserted deeply into the mitochondrial membrane, but only paepalantine dimer and vioxantin decreased the membrane's fluidity. A direct reaction with mitochondrial membrane protein thiols, involving an oxidation of these groups, is proposed to account for MPT induction by paepalantine, while a restriction of oxidation of these same thiol groups imposed by the decrease of membrane fluidity, is proposed to account for MPT inhibition by vioxanthin. (c) 2006 Published by Elsevier B.V..en
dc.format.extent155-164-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectisocoumarinspt
dc.subjectpaepalantinept
dc.subjectliver mitochondriapt
dc.subjectuncouplingpt
dc.subjectmitochondrial permeability transitionpt
dc.subjectpermeability transition porept
dc.subjectprotein thiol oxidationpt
dc.subjectmitochondrial membrane fluiditypt
dc.titleEffects of isocoumarins isolated from Paepalanthus bromelioides on mitochondria: Uncoupling, and induction/inhibition of mitochondrial permeability transitionen
dc.typeoutro-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv São Paulo, Fac Ciências Farmaceut, Dept Fis & Quim, BR-14040903 Ribeirao Preto, Brazil-
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, Araraquara, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Ciências Farmaceut, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, Araraquara, SP, Brazil-
dc.identifier.doi10.1016/j.cbi.2006.04.006-
dc.identifier.wosWOS:000238606700007-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofChemico-biological Interactions-
dc.identifier.orcid0000-0003-3032-2556pt
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.