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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/8022
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dc.contributor.authorMartins, Carlos Henrique Gomes-
dc.contributor.authorBauab, Taís Maria-
dc.contributor.authorLeite, Clarice Queico Fujimura-
dc.contributor.authorFalcão, Deise Pasetto-
dc.date.accessioned2014-05-20T13:25:21Z-
dc.date.available2014-05-20T13:25:21Z-
dc.date.issued2007-08-01-
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762007005000072-
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.-
dc.identifier.issn0074-0276-
dc.identifier.urihttp://hdl.handle.net/11449/8022-
dc.description.abstractRibotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.en
dc.format.extent587-592-
dc.language.isoeng-
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde-
dc.sourceSciELO-
dc.subjectribotypingen
dc.subjectYersinia pseudotuberculosisen
dc.subjectvirulence markersen
dc.subjectpolymerase chain reactionen
dc.titleRibotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazilen
dc.typeoutro-
dc.contributor.institutionUniversidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada-
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas-
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas-
dc.identifier.doi10.1590/S0074-02762007005000072-
dc.identifier.scieloS0074-02762007000500008-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileS0074-02762007000500008.pdf-
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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