Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/8701
- Title:
- Indicator-free electrochemical DNA hybridization biosensor
- New Mexico State Univ
- Univ Valencia
- Univ Nacl Cordoba
- Universidade Estadual Paulista (UNESP)
- 0003-2670
- A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a Aat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects. (C) 1998 Elsevier B.V. B.V. All rights reserved.
- 30-Nov-1998
- Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 375, n. 3, p. 197-203, 1998.
- 197-203
- Elsevier B.V.
- DNA hybridization
- biosensors
- guanine
- inosine
- carbon paste electrodes
- http://dx.doi.org/10.1016/S0003-2670(98)00503-0
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/8701
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