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http://acervodigital.unesp.br/handle/11449/8701
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DC Field | Value | Language |
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dc.contributor.author | Wang, J. | - |
dc.contributor.author | Rivas, G. | - |
dc.contributor.author | Fernandes, JR | - |
dc.contributor.author | Paz, JLL | - |
dc.contributor.author | Jiang, M. | - |
dc.contributor.author | Waymire, R. | - |
dc.date.accessioned | 2014-05-20T13:26:48Z | - |
dc.date.accessioned | 2016-10-25T16:46:53Z | - |
dc.date.available | 2014-05-20T13:26:48Z | - |
dc.date.available | 2016-10-25T16:46:53Z | - |
dc.date.issued | 1998-11-30 | - |
dc.identifier | http://dx.doi.org/10.1016/S0003-2670(98)00503-0 | - |
dc.identifier.citation | Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 375, n. 3, p. 197-203, 1998. | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/11449/8701 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/8701 | - |
dc.description.abstract | A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a Aat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects. (C) 1998 Elsevier B.V. B.V. All rights reserved. | en |
dc.format.extent | 197-203 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | DNA hybridization | pt |
dc.subject | biosensors | pt |
dc.subject | guanine | pt |
dc.subject | inosine | pt |
dc.subject | carbon paste electrodes | pt |
dc.title | Indicator-free electrochemical DNA hybridization biosensor | en |
dc.type | outro | - |
dc.contributor.institution | New Mexico State Univ | - |
dc.contributor.institution | Univ Valencia | - |
dc.contributor.institution | Univ Nacl Cordoba | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA | - |
dc.description.affiliation | Univ Valencia, Fac Ciências Quim, Dept Quim Analit, Valencia, Spain | - |
dc.description.affiliation | Univ Nacl Cordoba, Dept Quim Fis, RA-5000 Cordoba, Argentina | - |
dc.description.affiliation | UNESP, FC, Dept Quim, BR-17033360 Bauru, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, FC, Dept Quim, BR-17033360 Bauru, SP, Brazil | - |
dc.identifier.doi | 10.1016/S0003-2670(98)00503-0 | - |
dc.identifier.wos | WOS:000077069300001 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Analytica Chimica Acta | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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