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http://acervodigital.unesp.br/handle/11449/8703
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DC Field | Value | Language |
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dc.contributor.author | Menezes, Manoel Lima de | - |
dc.contributor.author | Demarchi, ACCO | - |
dc.date.accessioned | 2014-05-20T13:26:49Z | - |
dc.date.accessioned | 2016-10-25T16:46:53Z | - |
dc.date.available | 2014-05-20T13:26:49Z | - |
dc.date.available | 2016-10-25T16:46:53Z | - |
dc.date.issued | 1998-01-01 | - |
dc.identifier | http://dx.doi.org/10.1080/10826079808000543 | - |
dc.identifier.citation | Journal of Liquid Chromatography & Related Technologies. New York: Marcel Dekker Inc., v. 21, n. 15, p. 2355-2363, 1998. | - |
dc.identifier.issn | 1082-6076 | - |
dc.identifier.uri | http://hdl.handle.net/11449/8703 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/8703 | - |
dc.description.abstract | This method has been developed for extraction and determination of phenol in a urine sample by high performance liquid chromatography.After acid hydrolysis, the free phenol was extracted with isoamyl alcohol solvent, followed by back extraction with 0.5 mol.L-1 sodium hydroxide solution and analyzed by an isocratic HPLC Varian System, equipped with reverse-phase column (MicroPak-C-18). The mobile phase was acetonitrile in 0.01 mol.L-1 hydrochloric acid solution, (20:80 v/v), and at a now-rate of 1.0 mL.min-1. The chromatogram was monitored at 220 nm in room temperature. The identification was based on retention time and the quantification was performed by automatic peak-area determination, corrected for the external standards method.The recovery was higher than 99.5 % for phenol and reproducibility of method was shown to be 2.3% standard deviation and 5.6% coefficient of variance (n=20). The limit detection was 0.05 mgL(-1) and a range of 0.05 to 20.0 mgL(-1) of phenol for linearity. | en |
dc.format.extent | 2355-2363 | - |
dc.language.iso | eng | - |
dc.publisher | Marcel Dekker Inc | - |
dc.source | Web of Science | - |
dc.title | Off line extraction of phenol from human urine sample with isoamyl alcohol and determination by HPLC | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.description.affiliation | Univ Estadual Paulista, UNESP, Fac Ciências, Dept Quim, BR-17033360 Bauru, SP, Brazil | - |
dc.description.affiliation | Univ Sagrado Coracao, Lab Bioquim, BR-17044160 Bauru, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, UNESP, Fac Ciências, Dept Quim, BR-17033360 Bauru, SP, Brazil | - |
dc.identifier.doi | 10.1080/10826079808000543 | - |
dc.identifier.wos | WOS:000075691900008 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal of Liquid Chromatography & Related Technologies | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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