You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/8762
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDuarte, Roberta Aparecida-
dc.contributor.authorMello, Elaine Rodrigues-
dc.contributor.authorAraki, Camila-
dc.contributor.authorBolzani, Vanderlan da Silva-
dc.contributor.authorSiqueira e Silva, Dulce Helena-
dc.contributor.authorRegasini, Luis Octavio-
dc.contributor.authorAlves Silva, Tarsia Giabardo-
dc.contributor.authorCafundo de Morais, Mauro Cesar-
dc.contributor.authorXimenes, Valdecir Farias-
dc.contributor.authorSoares, Christiane Pienna-
dc.date.accessioned2014-05-20T13:26:56Z-
dc.date.accessioned2016-10-25T16:46:59Z-
dc.date.available2014-05-20T13:26:56Z-
dc.date.available2016-10-25T16:46:59Z-
dc.date.issued2010-10-01-
dc.identifierhttp://dx.doi.org/10.1007/s13277-010-0064-2-
dc.identifier.citationTumor Biology. Dordrecht: Springer, v. 31, n. 5, p. 513-522, 2010.-
dc.identifier.issn1010-4283-
dc.identifier.urihttp://hdl.handle.net/11449/8762-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/8762-
dc.description.abstractIn the present study, two alkaloids isolated from Pterogyne nitens, a plant native to Brazil, have been shown to induce apoptosis in human breast cancer cells. These compounds, pterogynine (PGN) and pterogynidine (PGD), were tested for their effect on a human infiltrating ductal carcinoma cell line (ZR-7531). The cell line was treated with each alkaloid at several concentrations. Time-dependence (with or without recuperation time) and concentration-dependence (in the range 0.25-10 mM) were investigated in cytotoxicity and apoptosis assays. The annexin assay indicated an apparently higher percentage of death by necrosis of malignant cells after 24 h exposure to both P. nitens extracts than the Hoechst assay. Thus, our results in the two tests demonstrated that the Hoechst assay can discriminate between late apoptotic cells and necrosis, whereas the flow cytometry-based annexin V assay cannot. We concluded that PGN and PGD have effective antineoplastic activity against human breast cancer cells in vitro, by inducing programmed cell death.en
dc.format.extent513-522-
dc.language.isoeng-
dc.publisherSpringer-
dc.sourceWeb of Science-
dc.subjectBreast cancer cell lineen
dc.subjectCytotoxic activityen
dc.subjectAlkaloidsen
dc.subjectApoptosisen
dc.subjectNecrosisen
dc.subjectFlow cytometryen
dc.titleAlkaloids extracted from Pterogyne nitens induce apoptosis in malignant breast cell lineen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv São Paulo State UNESP, Sch Pharmaceut Sci, BR-14801902 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo State UNESP, Inst Chem Araraquara, BR-14801970 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo State UNESP, Sch Sci Bauru, BR-17033360 São Paulo, Brazil-
dc.description.affiliationUnespUniv São Paulo State UNESP, Sch Pharmaceut Sci, BR-14801902 São Paulo, Brazil-
dc.description.affiliationUnespUniv São Paulo State UNESP, Inst Chem Araraquara, BR-14801970 São Paulo, Brazil-
dc.description.affiliationUnespUniv São Paulo State UNESP, Sch Sci Bauru, BR-17033360 São Paulo, Brazil-
dc.identifier.doi10.1007/s13277-010-0064-2-
dc.identifier.wosWOS:000282423500017-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofTumor Biology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.