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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/131313
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dc.contributor.authorSantos-Ebinuma, Valéria Carvalho-
dc.contributor.authorLopes, André Moreni-
dc.contributor.authorPessoa, Adalberto-
dc.contributor.authorTeixeira, Maria Francisca Simas-
dc.date.accessioned2015-12-07T15:33:45Z-
dc.date.accessioned2016-10-25T21:23:14Z-
dc.date.available2015-12-07T15:33:45Z-
dc.date.available2016-10-25T21:23:14Z-
dc.date.issued2015-
dc.identifierhttp://dx.doi.org/10.1002/btpr.2127-
dc.identifier.citationBiotechnology Progress, v. 31, n. 5, p. 1295-1304, 2015.-
dc.identifier.issn1520-6033-
dc.identifier.urihttp://hdl.handle.net/11449/131313-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131313-
dc.description.abstractSafety concerns related to the increasing and widespread application of synthetic coloring agents have increased the demand for natural colorants. Fungi have been employed in the production of novel and safer colorants. In order to obtain the colorants from fermented broth, suitable extraction systems must be developed. Aqueous two-phase polymer systems (ATPPS) offer a favorable chemical environment and provide a promising alternative for extracting and solubilizing these molecules. The aim of this study was to investigate the partitioning of red colorants from the fermented broth of Penicillium purpurogenum using an ATPPS composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA). Red colorants partitioned preferentially to the top (PEG-rich phase). In systems composed of PEG 6,000 g/mol/NaPA 8,000 g/mol, optimum colorant partition coefficient (KC ) was obtained in the presence of NaCl 0.1 M (KC  = 10.30) while the PEG 10,000 g/mol/NaPA 8,000 g/mol system in the presence of Na2 SO4 0.5 M showed the highest KC (14.78). For both polymers, the mass balance (%MB) and yield in the PEG phase (%ηTOP ) were close to 100 and 79%, respectively. The protein selectivity in all conditions evaluated ranged from 2.0-3.0, which shows a suitable separation of the red colorants and proteins present in the fermented broth. The results suggest that the partitioning of the red colorants is dependent on both the PEG molecular size and salt type. Furthermore, the results obtained support the potential application of ATPPS as the first step of a purification process to recover colorants from fermented broth of microorganisms.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.format.extent1295-1304-
dc.language.isoeng-
dc.publisherAmerican Institute of Chemical Engineers-
dc.sourcePubMed-
dc.subjectPenicillium purpurogenumen
dc.subjectAqueous two-phase polymer systemsen
dc.subjectExtractionen
dc.subjectPoly (ethylene glycol)en
dc.subjectRed colorantsen
dc.subjectSodium polyacrylateen
dc.titleExtraction of natural red colorants from the fermented broth of Penicillium purpurogenum using aqueous two-phase polymer systemsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Federal do Amazonas (UFAM)-
dc.description.affiliationDepartamento de Bioprocessos e Biotecnologia, Faculdade de Ciências Farmacêuticas (FCFAR), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil-
dc.description.affiliationDepartamento de Tecnologia Bioquímico-Farmacêutico, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo (USP), 05508-900 São Paulo, SP, Brasil-
dc.description.affiliationCulture Collection DPUA/UFAM, Universidade Federal do Amazonas (UFAM), 69077-000 Manaus, AM, Brasil-
dc.description.affiliationUnespDepartamento de Bioprocessos e Biotecnologia, Faculdade de Ciências Farmacêuticas (FCFAR), Universidade Estadual Paulista (UNESP), Araraquara, SP, Brasil-
dc.description.sponsorshipIdFAPESP: 2008/58280-0-
dc.description.sponsorshipIdFAPESP: 2013/08617-7-
dc.identifier.doi10.1002/btpr.2127-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiotechnology Progress-
dc.identifier.pubmed26097197-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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