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DC Field | Value | Language |
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dc.contributor.author | Cotrim, Camila Aparecida | - |
dc.contributor.author | Garrido, Saulo Santesso | - |
dc.contributor.author | Trovatti, Eliane | - |
dc.contributor.author | Marchetto, Reinaldo | - |
dc.date.accessioned | 2014-05-20T14:17:51Z | - |
dc.date.accessioned | 2016-10-25T17:40:11Z | - |
dc.date.available | 2014-05-20T14:17:51Z | - |
dc.date.available | 2016-10-25T17:40:11Z | - |
dc.date.issued | 2010-01-01 | - |
dc.identifier | http://dx.doi.org/10.1590/S0100-40422010000400014 | - |
dc.identifier.citation | Química Nova. Sociedade Brasileira de Química, v. 33, n. 4, p. 841-845, 2010. | - |
dc.identifier.issn | 0100-4042 | - |
dc.identifier.uri | http://hdl.handle.net/11449/25350 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/25350 | - |
dc.description.abstract | Toxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11.7 kDa), preventing the cytotoxic activity of CcdB on the DNA gyrase. As an approach to understanding this interaction, CcdA41, a polypeptide derived from CcdA, was synthesized by solid-phase methodology and its interaction with CcdB was analyzed by steady state fluorescence. CcdA41 formed a stable complex with CcdBET2, a peptide based on CcdB, the more recently described bacterial topoisomerase inhibitor. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 841-845 | - |
dc.language.iso | por | - |
dc.publisher | Sociedade Brasileira de Química | - |
dc.source | SciELO | - |
dc.subject | bacterial toxin | en |
dc.subject | peptides | en |
dc.subject | fluorescence | en |
dc.title | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário | pt |
dc.title.alternative | Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Departamento de Bioquímica e Tecnologia Química, Instituto de Química, Universidade Estadual Paulista, CP 355, 14800-900 Araraquara - SP, Brasil | - |
dc.description.affiliationUnesp | Departamento de Bioquímica e Tecnologia Química, Instituto de Química, Universidade Estadual Paulista, CP 355, 14800-900 Araraquara - SP, Brasil | - |
dc.description.sponsorshipId | FAPESP: 03/04492-3 | - |
dc.description.sponsorshipId | FAPESP: 07/08052-9 | - |
dc.identifier.doi | 10.1590/S0100-40422010000400014 | - |
dc.identifier.scielo | S0100-40422010000400014 | - |
dc.identifier.wos | WOS:000278322500014 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | S0100-40422010000400014.pdf | - |
dc.relation.ispartof | Química Nova | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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