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dc.contributor.authorCardoso, Tereza Cristina-
dc.contributor.authorMontassier, Hélio José-
dc.contributor.authorGalletti, Maria Do Carmo M.-
dc.contributor.authorPinto, Aramis Augusto-
dc.identifier.citationRevista de Microbiologia, v. 27, n. 1, p. 64-69, 1996.-
dc.description.abstractAn indirect ELISA method was developed and applied to detect antibodies against infectious bronchitis virus (IBV). Three hundred serum samples collected from vaccinated and non-vaccinated chickens under field conditions were tested. The ELISA results were compared with antibody titers determined by a serum neutralization assay (SN). Good coefficients of correlation (r2 = 0.750) and of agreement (80%) were recorded and the ELISA reproducibility was high. The cutoff point between those techniques corresponded to ELISA level 2. It is concluded that our indirect ELISA method can advantageously replace the conventional SN test, allowing a good follow-up of IBV post-vaccination antibodies due to its convenience and applicability compared to the other assay.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.subjectInfectious Bronchitis Virus (IBV)-
dc.subjectPost-vaccine serological monitoring-
dc.titleEvaluation of an indirect ELISA method for the detection of chicken antibodies against infectious bronchitis virusen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionSOLVAY Saúde Animal-
dc.description.affiliationCAPES MEC, Brasília-DF-
dc.description.affiliationFaculdade Cie. Agrarias V. UNESP Departamento de Microbiologia, 14870-000 Jaboticabal-SP-
dc.description.affiliationSOLVAY Saúde Animal, Campinas São Paulo-
dc.description.affiliationUnespFaculdade Cie. Agrarias V. UNESP Departamento de Microbiologia, 14870-000 Jaboticabal-SP-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofRevista de Microbiologia-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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